RRC ID 11274
著者 Nakahara Y, Shimura S, Ueno C, Kanamori Y, Mita K, Kiuchi M, Kamimura M.
タイトル Purification and characterization of silkworm hemocytes by flow cytometry.
ジャーナル Dev Comp Immunol
Abstract Hemocyte functions are well-investigated in the silkworm, Bombyx mori, however, detailed analysis of each hemocyte subset has been hampered by the lack of appropriate separation method. Here we use an array of flow cytometric analyses to characterize silkworm hemocytes with various molecular probes, such as propidium iodide, green fluorescence protein, monoclonal antibodies, and fluorescent lectins. Of these, separation using propidium iodide was the simplest and provided most reliable results for the isolation of the hemocyte subsets. cDNAs were then synthesized from these sorted populations and subset-specific gene expression was examined by RT-PCR. Granulocytes, plasmatocytes, and oenocytoids expressed different classes of immune genes, suggesting that they have multiple roles in silkworm immunity. In contrast, a contribution of spherulocytes to immunity was not documented in that they failed to express most of the genes. The functions of spherulocytes are thus likely to be distinct from those of the other three hemocyte subsets.
巻・号 33(4)
ページ 439-48
公開日 2009-4-1
DOI 10.1016/j.dci.2008.09.005
PII S0145-305X(08)00202-4
PMID 18840462
MeSH Animals Antibodies, Monoclonal Bombyx / cytology Bombyx / genetics Bombyx / immunology* Cell Separation Flow Cytometry Gene Expression Profiling Hemocytes / cytology* Hemocytes / immunology* Oligonucleotide Array Sequence Analysis Plant Lectins / metabolism
IF 3.192
引用数 29
WOS 分野 ZOOLOGY IMMUNOLOGY
リソース情報
カイコ cDNA clones