RRC ID 11278
Author Karasaki N, Mon H, Takahashi M, Lee JM, Koga K, Kawaguchi Y, Kusakabe T.
Title Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori.
Journal Biotechnol. Lett.
Abstract Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 x tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
Volume 31(4)
Pages 495-500
Published 2009-4
DOI 10.1007/s10529-008-9898-8
PMID 19066730
MeSH Animals Antigens, Polyomavirus Transforming / genetics Bombyx* Doxycycline / metabolism* Gene Expression* Molecular Biology / methods* Nucleopolyhedrovirus / genetics Promoter Regions, Genetic Simian virus 40 / genetics Transcriptional Activation*
IF 1.846
Times Cited 5
Silkworms ?