RRC ID 11278
著者 Karasaki N, Mon H, Takahashi M, Lee JM, Koga K, Kawaguchi Y, Kusakabe T.
タイトル Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori.
ジャーナル Biotechnol Lett
Abstract Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 x tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
巻・号 31(4)
ページ 495-500
公開日 2009-4-1
DOI 10.1007/s10529-008-9898-8
PMID 19066730
MeSH Animals Antigens, Polyomavirus Transforming / genetics Bombyx* Doxycycline / metabolism* Gene Expression* Molecular Biology / methods* Nucleopolyhedroviruses / genetics Promoter Regions, Genetic Simian virus 40 / genetics Transcriptional Activation*
IF 1.977
引用数 5
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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