RRC ID |
11278
|
Author |
Karasaki N, Mon H, Takahashi M, Lee JM, Koga K, Kawaguchi Y, Kusakabe T.
|
Title |
Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori.
|
Journal |
Biotechnol Lett
|
Abstract |
Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 x tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
|
Volume |
31(4)
|
Pages |
495-500
|
Published |
2009-4-1
|
DOI |
10.1007/s10529-008-9898-8
|
PMID |
19066730
|
MeSH |
Animals
Antigens, Polyomavirus Transforming / genetics
Bombyx*
Doxycycline / metabolism*
Gene Expression*
Molecular Biology / methods*
Nucleopolyhedroviruses / genetics
Promoter Regions, Genetic
Simian virus 40 / genetics
Transcriptional Activation*
|
IF |
1.977
|
Times Cited |
5
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
Resource |
Silkworms |
|