RRC ID |
11278
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著者 |
Karasaki N, Mon H, Takahashi M, Lee JM, Koga K, Kawaguchi Y, Kusakabe T.
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タイトル |
Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori.
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ジャーナル |
Biotechnol Lett
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Abstract |
Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 x tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
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巻・号 |
31(4)
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ページ |
495-500
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公開日 |
2009-4-1
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DOI |
10.1007/s10529-008-9898-8
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PMID |
19066730
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MeSH |
Animals
Antigens, Polyomavirus Transforming / genetics
Bombyx*
Doxycycline / metabolism*
Gene Expression*
Molecular Biology / methods*
Nucleopolyhedroviruses / genetics
Promoter Regions, Genetic
Simian virus 40 / genetics
Transcriptional Activation*
|
IF |
1.977
|
引用数 |
5
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WOS 分野
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BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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リソース情報 |
カイコ |
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