RRC ID 11280
Author Yoshido A, Yasukochi Y, Sahara K.
Title Samia cynthia versus Bombyx mori: comparative gene mapping between a species with a low-number karyotype and the model species of Lepidoptera.
Journal Insect Biochem. Mol. Biol.
Abstract We performed gene-based comparative FISH mapping between a wild silkmoth, Samia cynthia ssp. with a low number of chromosomes (2n=25-28) and the model species, Bombyx mori (2n=56), in order to identify the genomic components that make up the chromosomes in a low-number karyotype. Mapping of 64 fosmid probes containing orthologs of B. mori genes revealed that the homologues of either two or four B. mori chromosomes constitute the S. c. ricini (Vietnam population, 2n=27♀/28♂, Z0/ZZ) autosomes. Where tested, even the gene order was conserved between S. c. ricini and B. mori. This was also true for the originally autosomal parts of the neo-sex chromosomes in S. c. walkeri (Sapporo population, 2n=26♀/26♂, neo-Wneo-Z/neo-Zneo-Z) and S. cynthia subsp. indet. (Nagano population, 2n=25♀/26♂, neo-WZ₁Z₂/Z₁Z₁Z₂Z₂). The results are evidence for an internal stability of lepidopteran chromosomes even when all autosomes had undergone fusion processes to form a low-number karyotype.
Volume 41(6)
Pages 370-7
Published 2011-6
DOI 10.1016/j.ibmb.2011.02.005
PII S0965-1748(11)00053-1
PMID 21396446
MeSH Animals Cell Nucleus / genetics* Cell Nucleus / ultrastructure Chromosome Mapping* Cloning, Molecular Escherichia coli Female Gene Expression Gene Library Gene Order Genes, Insect* Genetic Linkage Genome Genomic Instability In Situ Hybridization, Fluorescence Japan Karyotyping Moths / cytology Moths / genetics* Recombinant Proteins / genetics Recombinant Proteins / metabolism* Sex Chromosomes / genetics* Sex Chromosomes / ultrastructure Species Specificity Vietnam
IF 3.562
Times Cited 10
Silkworms NA