RRC ID 11283
著者 Kokuho T, Yasukochi Y, Watanabe S, Inumaru S.
タイトル Molecular cloning and expression profile analysis of a novel beta-D-N-acetylhexosaminidase of domestic silkworm (Bombyx mori).
ジャーナル Genes Cells
Abstract Lepidoptera such as the domestic silkworm (Bombyx mori) produce proteins modified with unsialylated, mannose-rich moieties known as 'high mannose-type'N-glycans. However, we observed that, under intrinsic acetylglucosaminidase (GlcNAcase)-inhibited conditions, moth cells tend to synthesize different types of glycoform with sialic acid modification. To identify molecules essential to assemble Lepidoptera-specific N-glycans, we performed BLAST analysis on the silkworm genetic database and isolated the entire coding sequence of novel Bombyx GlcNAcase, BmGlcNAcase 2. This enzyme showed weak homology to currently known, lysosome-associated eukaryotic hexosaminidases, but it revealed remarkable similarity with recently reported glycosyl hydrolases of Spodoptera and Bombyx. Interestingly, BmGlcNAcase 2 was found to be expressed in embryos and in certain tissues of molting larvae (i.e. ovary, fat bodies, mid-intestine, skin), but not in pupae, suggesting its unique function in the carbohydrate metabolism of juvenile silkworm.
巻・号 15(5)
ページ 525-36
公開日 2010-5-1
DOI 10.1111/j.1365-2443.2010.01401.x
PII GTC1401
PMID 20384789
MeSH Amino Acid Sequence Animals Bombyx / enzymology* Bombyx / genetics Cloning, Molecular Gene Expression Profiling Insect Proteins / classification Insect Proteins / genetics* Insect Proteins / metabolism Isoenzymes / classification Isoenzymes / genetics* Isoenzymes / metabolism Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Amino Acid beta-N-Acetylhexosaminidases / classification beta-N-Acetylhexosaminidases / genetics* beta-N-Acetylhexosaminidases / metabolism
IF 1.655
引用数 5
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
カイコ EST database