| RRC ID |
11293
|
| 著者 |
Mitsumasu K, Ohta H, Tsuchihara K, Asaoka K, Ozoe Y, Niimi T, Yamashita O, Yaginuma T.
|
| タイトル |
Molecular cloning and characterization of cDNAs encoding dopamine receptor-1 and -2 from brain-suboesophageal ganglion of the silkworm, Bombyx mori.
|
| ジャーナル |
Insect Mol Biol
|
| Abstract |
In order to better understand the relationship between dopamine and the release of diapause hormone into the blood, we cloned and characterized cDNAs encoding Bombyx mori dopamine receptor-1 and -2 (BmDopR1 and 2) from the pupal brain-suboesophageal ganglion. BmDopR1 and 2 had high similarities to group 1 (Drosophila melanogaster DOP1 and Apis mellifera DOP1) and group 2 (D. melanogaster DopR99B, A. mellifera DOP2 and Papilio xuthus DOP1), respectively. When BmDopR1 and 2 were expressed in human embryonic kidney (HEK) cells, they responded to dopamine by increasing intracellular cAMP levels, thus indicating the presence of D1-like receptors. There were no clear differences in BmDopR1 and 2 mRNA levels between brain-suboesophageal ganglion complexes of diapause and nondiapause egg producers during pupal-adult development. BmDopR1 and 2 mRNAs were concentrated in the mushroom body calyx rather than in the suboesophageal ganglion. Taking into account the results of earlier experiments on excised regions corresponding to mushroom bodies, BmDopR1 and 2 in the mushroom body apparently play a role in the release of diapause hormone.
|
| 巻・号 |
17(2)
|
| ページ |
185-95
|
| 公開日 |
2008-4-1
|
| DOI |
10.1111/j.1365-2583.2008.00792.x
|
| PII |
IMB792
|
| PMID |
18353107
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Bombyx / genetics*
Brain / metabolism
Cell Line
Cloning, Molecular
DNA, Complementary / genetics
Female
Ganglia, Invertebrate / chemistry
Ganglia, Invertebrate / metabolism
Humans
In Situ Hybridization
Molecular Sequence Data
Neuropeptides / physiology
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Receptors, Dopamine D1 / genetics*
Receptors, Dopamine D2 / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Transfection
|
| IF |
2.533
|
| 引用数 |
18
|
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
|
