論文 - 詳細
| RRC ID | 11309 |
|---|---|
| 著者 | Manaboon M, Iga M, Iwami M, Sakurai S. |
| タイトル | Intracellular mobilization of Ca2+ by the insect steroid hormone 20-hydroxyecdysone during programmed cell death in silkworm anterior silk glands. |
| ジャーナル | J Insect Physiol |
| Abstract |
20-Hydroxyecdysone (20E) triggers programmed cell death (PCD) and regulates de novo gene expression in the anterior silk glands (ASGs) of the silkworm Bombyx mori. PCD is mediated via a nongenomic pathway that includes Ca2+ as a second messenger and the activation of protein kinase C/caspase-3-like protease; however, the steps leading to a concomitant buildup of intracellular Ca2+ are unknown. We employed pharmacological tools to identify the components of this pathway. ASGs were cultured in the presence of 1 microM 20E and one of the following inhibitors: a G-protein-coupled receptor (GPCR) inhibitor, a phospholipase C (PLC) inhibitor, an inositol 1,4,5-trisphosphate receptor (IP3R) antagonist, and an L- or T-type Ca2+ channel blocker. The T-type Ca2+ channel blocker inhibited 20E-induced nuclear and DNA fragmentation; in contrast, PCD was induced by 20E in Ca2+-free medium, indicating that the source of Ca2+ is an intracellular reservoir. The IP3R antagonist inhibited nuclear and DNA fragmentation, suggesting that the endoplasmic reticulum may be the Ca2+ source. Finally, the GPCR and PLC inhibitors effectively blocked nuclear and DNA fragmentation. Our results indicate that 20E increases the intracellular level of Ca2+ by activating IP3R, and that this effect may be brought about by the serial activation of GPCR, PLC, and IP3. |
| 巻・号 | 55(2) |
| ページ | 122-8 |
| 公開日 | 2009-2-1 |
| DOI | 10.1016/j.jinsphys.2008.10.013 |
| PII | S0022-1910(08)00226-6 |
| PMID | 19041319 |
| MeSH | Animals Apoptosis / physiology* Bombyx / metabolism* Calcium / metabolism* DNA Fragmentation Ecdysterone / metabolism* Exocrine Glands / metabolism Indoles Second Messenger Systems / physiology* |
| IF | 2.246 |
| 引用数 | 20 |
| WOS 分野 | ENTOMOLOGY ZOOLOGY PHYSIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | NA |