RRC ID 11309
著者 Manaboon M, Iga M, Iwami M, Sakurai S.
タイトル Intracellular mobilization of Ca2+ by the insect steroid hormone 20-hydroxyecdysone during programmed cell death in silkworm anterior silk glands.
ジャーナル J Insect Physiol
Abstract 20-Hydroxyecdysone (20E) triggers programmed cell death (PCD) and regulates de novo gene expression in the anterior silk glands (ASGs) of the silkworm Bombyx mori. PCD is mediated via a nongenomic pathway that includes Ca2+ as a second messenger and the activation of protein kinase C/caspase-3-like protease; however, the steps leading to a concomitant buildup of intracellular Ca2+ are unknown. We employed pharmacological tools to identify the components of this pathway. ASGs were cultured in the presence of 1 microM 20E and one of the following inhibitors: a G-protein-coupled receptor (GPCR) inhibitor, a phospholipase C (PLC) inhibitor, an inositol 1,4,5-trisphosphate receptor (IP3R) antagonist, and an L- or T-type Ca2+ channel blocker. The T-type Ca2+ channel blocker inhibited 20E-induced nuclear and DNA fragmentation; in contrast, PCD was induced by 20E in Ca2+-free medium, indicating that the source of Ca2+ is an intracellular reservoir. The IP3R antagonist inhibited nuclear and DNA fragmentation, suggesting that the endoplasmic reticulum may be the Ca2+ source. Finally, the GPCR and PLC inhibitors effectively blocked nuclear and DNA fragmentation. Our results indicate that 20E increases the intracellular level of Ca2+ by activating IP3R, and that this effect may be brought about by the serial activation of GPCR, PLC, and IP3.
巻・号 55(2)
ページ 122-8
公開日 2009-2-1
DOI 10.1016/j.jinsphys.2008.10.013
PII S0022-1910(08)00226-6
PMID 19041319
MeSH Animals Apoptosis / physiology* Bombyx / metabolism* Calcium / metabolism* DNA Fragmentation Ecdysterone / metabolism* Exocrine Glands / metabolism Indoles Second Messenger Systems / physiology*
IF 2.246
引用数 20
WOS 分野 ENTOMOLOGY ZOOLOGY PHYSIOLOGY
リソース情報
カイコ NA