RRC ID 11320
Author Hashimoto K, Yamano Y, Morishima I.
Title Induction of tyrosine hydroxylase gene expression by bacteria in the fat body of eri-silkworm, Samia cynthia ricini.
Journal Comp Biochem Physiol B Biochem Mol Biol
Abstract A cDNA clone encoding tyrosine hydroxylase (TH) was isolated from larval fat body of immunized Samia cynthia ricini. In naive larvae, the TH gene was expressed only in the brain, but strongly induced in fat body and hemocytes after injecting UV-killed bacteria. The induction of the gene was rather short-lived compared to that of antibacterial protein genes, reaching the maximum levels 6h after bacterial challenge, and then quickly diminished. A strong induction of the gene expression was caused by both Gram-negative and positive bacteria and zymosan, but little if any by soluble peptidoglycan or lipopolysaccharide. A possible role of TH in the fat body of bacteria-challenged larvae would be to supply catecholamines as the substrate for phenoloxidase leading to melanization, working together with dopa decarboxylase.
Volume 149(3)
Pages 501-6
Published 2008-3-1
DOI 10.1016/j.cbpb.2007.11.010
PII S1096-4959(07)00440-X
PMID 18178496
MeSH Amino Acid Sequence Animals Bacteria / metabolism* Base Sequence Bombyx / enzymology* Bombyx / microbiology* Cell Wall / metabolism Clone Cells DNA, Complementary / isolation & purification Enzyme Induction Fat Body / enzymology* Fat Body / microbiology* Gene Expression Profiling Molecular Sequence Data Organ Specificity Sequence Alignment Time Factors Tyrosine 3-Monooxygenase / biosynthesis* Tyrosine 3-Monooxygenase / chemistry Tyrosine 3-Monooxygenase / genetics
IF 2.219
Times Cited 8
Silkworms NA