論文 - 詳細
| RRC ID | 11326 |
|---|---|
| 著者 | Tanaka H, Suzuki N, Nakajima Y, Sato M, Sagisaka A, Fujita K, Ishibashi J, Imanishi S, Mita K, Yamakawa M. |
| タイトル | Expression profiling of novel bacteria-induced genes from the silkworm, Bombyx mori. |
| ジャーナル | Arch Insect Biochem Physiol |
| Abstract |
In this study, we have newly identified three bacteria-induced genes from the silkworm Bombyx mori by quantitative reverse transcriptase-polymerase chain reaction. One of these, eukaryotic initiation factor 4E-1 (eIF4E-1), is assumed to encode an eIF4E family, which plays a role in the initiation of translation as a mRNA cap-binding protein. The second gene is BmFOXG1, belonging to a family of forkhead transcription factors, FOXG1. The third gene is MBF2-related (MBF2-R) whose product has high homology to a co-activator protein MBF2 from B. mori. Although BmFOXG1 was up-regulated in the fat body in response to three kinds of bacteria, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, eIF4E-1 and MBF2-R were up-regulated by E. coli and B. subtilis, but not S. aureus, suggesting that bacteria possessing meso-diaminopimelic acid-containing peptidoglycan but not lysine-containing peptidoglycan activate eIF4E-1 and MBF2-R, probably through a conserved immune deficiency pathway. We further profiled the expression of three genes in different tissues and a silkworm cell line, NIAS-Bm-aff3, in response to bacteria, and at different times after bacterial challenge in the fat body. |
| 巻・号 | 73(3) |
| ページ | 148-62 |
| 公開日 | 2010-3-1 |
| DOI | 10.1002/arch.20347 |
| PMID | 20077574 |
| MeSH | Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Bombyx / metabolism* Bombyx / microbiology Cell Line Escherichia coli / physiology Fat Body / metabolism Gene Expression Profiling* Genes, Insect / genetics* Insect Proteins / chemistry Insect Proteins / genetics Insect Proteins / metabolism Larva / genetics Larva / metabolism Larva / microbiology Molecular Sequence Data Sequence Alignment Sequence Homology, Amino Acid Up-Regulation |
| IF | 1.536 |
| 引用数 | 9 |
| WOS 分野 | ENTOMOLOGY PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 各媒体での言及数の合計 | 0 |
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