RRC ID 11326
著者 Tanaka H, Suzuki N, Nakajima Y, Sato M, Sagisaka A, Fujita K, Ishibashi J, Imanishi S, Mita K, Yamakawa M.
タイトル Expression profiling of novel bacteria-induced genes from the silkworm, Bombyx mori.
ジャーナル Arch Insect Biochem Physiol
Abstract In this study, we have newly identified three bacteria-induced genes from the silkworm Bombyx mori by quantitative reverse transcriptase-polymerase chain reaction. One of these, eukaryotic initiation factor 4E-1 (eIF4E-1), is assumed to encode an eIF4E family, which plays a role in the initiation of translation as a mRNA cap-binding protein. The second gene is BmFOXG1, belonging to a family of forkhead transcription factors, FOXG1. The third gene is MBF2-related (MBF2-R) whose product has high homology to a co-activator protein MBF2 from B. mori. Although BmFOXG1 was up-regulated in the fat body in response to three kinds of bacteria, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, eIF4E-1 and MBF2-R were up-regulated by E. coli and B. subtilis, but not S. aureus, suggesting that bacteria possessing meso-diaminopimelic acid-containing peptidoglycan but not lysine-containing peptidoglycan activate eIF4E-1 and MBF2-R, probably through a conserved immune deficiency pathway. We further profiled the expression of three genes in different tissues and a silkworm cell line, NIAS-Bm-aff3, in response to bacteria, and at different times after bacterial challenge in the fat body.
巻・号 73(3)
ページ 148-62
公開日 2010-3-1
DOI 10.1002/arch.20347
PMID 20077574
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Bombyx / metabolism* Bombyx / microbiology Cell Line Escherichia coli / physiology Fat Body / metabolism Gene Expression Profiling* Genes, Insect / genetics* Insect Proteins / chemistry Insect Proteins / genetics Insect Proteins / metabolism Larva / genetics Larva / metabolism Larva / microbiology Molecular Sequence Data Sequence Alignment Sequence Homology, Amino Acid Up-Regulation
IF 1.536
引用数 9
WOS 分野 ENTOMOLOGY PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
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