RRC ID 1133
Author Wauters G, Avesani V, Charlier J, Janssens M, Vaneechoutte M, Delmée M.
Title Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory.
Journal J. Clin. Microbiol.
Abstract Eighty-six Nocardia strains isolated from clinical samples in Belgium were identified by 16S rRNA gene sequencing. Eighty-three (96%) strains belonged to only six Nocardia species: N. farcinica (38 [44%]), N. nova (19 [22%]), N. cyriacigeorgica (13 [15%]), N. brasiliensis (6 [6.9%]), N. abscessus (5 [5.8%]), and N. paucivorans (2 [2.3%]). A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme.
Volume 43(6)
Pages 2624-8
Published 2005-6
DOI 10.1128/JCM.43.6.2624-2628.2005
PII 43/6/2624
PMID 15956375
PMC PMC1151960
MeSH Algorithms Bacterial Typing Techniques Belgium / epidemiology DNA, Bacterial / analysis DNA, Ribosomal / analysis Genes, rRNA Humans Nocardia / classification* Nocardia / enzymology Nocardia / genetics Nocardia / isolation & purification* Nocardia Infections / epidemiology* Nocardia Infections / microbiology RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Species Specificity Time Factors
IF 4.959
Times Cited 50
Pathogenic microorganisms ?