RRC ID 1135
Author Sugita T, Takeo K, Hama K, Virtudazo E, Takashima M, Nishikawa A, Kucsera J, Dorogi J, Komori S, Nakagaki K, Vollekova A, Slavikova E, Farkas V.
Title DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals.
Journal Med. Mycol.
Abstract The non-lipid-dependent species Malassezia pachydermatis is frequently isolated from animals. We analyzed the DNA sequences of the intergenic spacer (IGS) 1 region, which is the most variable region in the rRNA gene, of 43 M. pachydermatis strains obtained from dogs or cats. The lengths of the IGS 1 regions ranged from 552 to 898 bp and, based on the nucleotide sequence, these IGS 1 regions were divided into three major groups with 10 subtypes. Group 1 (552-601 bp long) was characterized by the short sequence repeat (CAGCA)n and had four to 14 repeats, and Group 3 (749-898 bp long), which included the neotype strain of M. pachydermatis, was characterized by the sequence (CAGCATAACATAACACACAACA)n in the IGS1 region. Group 2 possessed partial sequences of both Groups 1 and 3. Each group shared only 41.7-55.4% similarity in the IGS1 region with the other groups. The internal transcribed spacer (ITS) region and D1/D2 26S rDNA in the rRNA gene were also sequenced for representative strains in each IGS group. The groups were distinguished by both ITS (698-712 bp long including 5.8S rDNA) and D1/D2 26S rDNA (624 bp long) sequences with sequence similarities of 91.7-96.0% and 99.7-99.0%, respectively. Our results indicate that the sequence of the IGS region of M. pachydermatis has a remarkable intraspecies diversity, compared with ITS or D1/D2 26S rDNA, and that multiple genotypic strains of M. pachydermatis colonize animal skin.
Volume 43(1)
Pages 21-6
Published 2005-2
PMID 15712605
MeSH Animals Base Sequence Cat Diseases / microbiology* Cats DNA, Fungal / analysis DNA, Ribosomal Spacer / analysis DNA, Ribosomal Spacer / genetics* Dermatomycoses / microbiology Dermatomycoses / veterinary* Dog Diseases / microbiology* Dogs Genetic Variation* Malassezia / classification* Malassezia / genetics Molecular Sequence Data RNA, Ribosomal / genetics Sequence Analysis, DNA Skin / microbiology
IF 2.851
Times Cited 28
Pathogenic microorganisms IFM 52734 IFM 52735 IFM 52736 IFM 52737 IFM 52738 IFM 52739 IFM 52740 IFM 52741 IFM 52742 IFM 52743 IFM 52744 IFM 52745 IFM 52746 IFM 52747 IFM 52748 IFM 52749 IFM 52750 IFM 52751 IFM 52752 IFM 52753 IFM 52754 IFM 52755 IFM 52757 IFM 52758 IFM 52759 IFM 52760 IFM 52761 IFM 52762 IFM 52763 IFM 52764 IFM 52765 IFM 52766 IFM 52767 IFM 52768 IFM 52769 IFM 52770 IFM 52771 IFM 52772 IFM 52773 IFM 52774 IFM 52775 IFM 52776 M. pachydermatis?