RRC ID |
11353
|
Author |
Kambe Y, Takeda Y, Yamamoto K, Kojima K, Tamada Y, Tomita N.
|
Title |
Effect of RGDS-expressing fibroin dose on initial adhesive force of a single chondrocyte.
|
Journal |
Biomed Mater Eng
|
Abstract |
Initial chondrocyte-material interactions are important for cell behaviors such as proliferation, phenotypic expression and matrix synthesis. Previously, we showed that chondrocytes cultured in/on silk fibroin scaffolds proliferate without dedifferentiating into fibroblast-like cells and that RGDS sequences genetically interfused in the fibroin light chain protein enhance cartilage tissue formation. In the present study, the adhesive force of chondrocytes was measured on fibroin substrates containing RGDS-expressing fibroin molecules produced by transgenic silkworms at the different densities of 0, 0.6, 1.5 and 3.0 mol%. The degree of chondrocyte attachment to fibroin substrates increased with the number of RGDS-expressing fibroin molecules. Moreover, the adhesive force per unit spreading area of a single cultured chondrocyte exhibited a peak that was higher with increased RGDS concentrations. The results of this study indicate that the RGDS sequences genetically interfused in the fibroin light chain protein exert effects on chondrocytes' adhesive behavior and can enhance cartilage tissue organization.
|
Volume |
20(6)
|
Pages |
309-16
|
Published |
2010-1-1
|
DOI |
10.3233/BME-2010-0644
|
PII |
FK0W1511220GP474
|
PMID |
21263177
|
MeSH |
Adhesiveness
Animals
Animals, Genetically Modified
Bombyx / genetics
Bombyx / metabolism
Cell Adhesion / drug effects
Cell Culture Techniques / instrumentation
Cells, Cultured
Chondrocytes / drug effects*
Chondrocytes / metabolism
Chondrocytes / physiology*
Coated Materials, Biocompatible / chemistry
Coated Materials, Biocompatible / pharmacology
Dose-Response Relationship, Drug
Fibroins / chemistry
Fibroins / genetics
Fibroins / metabolism
Fibroins / pharmacology*
Male
Oligopeptides / chemistry*
Oligopeptides / genetics
Oligopeptides / pharmacology
Rabbits
Surface Properties
Tissue Scaffolds / chemistry
Transfection
|
IF |
1.243
|
Times Cited |
7
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
Resource |
Silkworms |
NA |