RRC ID 11353
Author Kambe Y, Takeda Y, Yamamoto K, Kojima K, Tamada Y, Tomita N.
Title Effect of RGDS-expressing fibroin dose on initial adhesive force of a single chondrocyte.
Journal Biomed Mater Eng
Abstract Initial chondrocyte-material interactions are important for cell behaviors such as proliferation, phenotypic expression and matrix synthesis. Previously, we showed that chondrocytes cultured in/on silk fibroin scaffolds proliferate without dedifferentiating into fibroblast-like cells and that RGDS sequences genetically interfused in the fibroin light chain protein enhance cartilage tissue formation. In the present study, the adhesive force of chondrocytes was measured on fibroin substrates containing RGDS-expressing fibroin molecules produced by transgenic silkworms at the different densities of 0, 0.6, 1.5 and 3.0 mol%. The degree of chondrocyte attachment to fibroin substrates increased with the number of RGDS-expressing fibroin molecules. Moreover, the adhesive force per unit spreading area of a single cultured chondrocyte exhibited a peak that was higher with increased RGDS concentrations. The results of this study indicate that the RGDS sequences genetically interfused in the fibroin light chain protein exert effects on chondrocytes' adhesive behavior and can enhance cartilage tissue organization.
Volume 20(6)
Pages 309-16
Published 2010-1-1
DOI 10.3233/BME-2010-0644
PII FK0W1511220GP474
PMID 21263177
MeSH Adhesiveness Animals Animals, Genetically Modified Bombyx / genetics Bombyx / metabolism Cell Adhesion / drug effects Cell Culture Techniques / instrumentation Cells, Cultured Chondrocytes / drug effects* Chondrocytes / metabolism Chondrocytes / physiology* Coated Materials, Biocompatible / chemistry Coated Materials, Biocompatible / pharmacology Dose-Response Relationship, Drug Fibroins / chemistry Fibroins / genetics Fibroins / metabolism Fibroins / pharmacology* Male Oligopeptides / chemistry* Oligopeptides / genetics Oligopeptides / pharmacology Rabbits Surface Properties Tissue Scaffolds / chemistry Transfection
IF 1.243
Times Cited 7
Silkworms NA