Reference - Detail
|Author||Chikamori M, Fukushima K.|
|Title||A new hexose transporter from Cryptococcus neoformans: molecular cloning and structural and functional characterization.|
|Journal||Fungal Genet. Biol.|
We carried out a screen for Cryptococcus neoformans genes involved in resistance to copper ion toxicity and identified a new hexose transporter (Hxt) gene, HXT1. Hxt1 consists of 520 amino acids and functions to transport hexoses such as glucose. Although Hxt1 conferred copper resistance to Saccharomyces cerevisiae, disruption of the HXT1 gene showed that Hxt1 is not necessary for copper resistance. In virulence tests, an hxt1 mutant strain showed 12% less phenoloxidase activity than the wild-type strain, and no difference in the ability to form melanin was identified. In addition, the hxt1 mutant strain showed virulence similar to that of the wild-type strain in experiments with Caenorhabditis elegans. However, the hxt1 mutant strain generated larger capsules than were generated by the wild-type strain. Thus, Hxt1 appears to be involved in capsule formation.
|MeSH||Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / growth & development Cloning, Molecular* Copper / pharmacology* Copper / toxicity Cryptococcus neoformans / drug effects* Cryptococcus neoformans / genetics Cryptococcus neoformans / pathogenicity DNA, Complementary Drug Resistance, Fungal / drug effects Drug Resistance, Fungal / genetics* Fungal Proteins / genetics Fungal Proteins / metabolism Gene Expression Regulation, Fungal Monosaccharide Transport Proteins / genetics* Monosaccharide Transport Proteins / metabolism* Mutation Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Virulence|
|WOS Category||MYCOLOGY GENETICS & HEREDITY|
|Pathogenic microorganisms||C. neoformans?|