RRC ID 11368
著者 Takada H, Nishida A, Domae M, Kita A, Yamano Y, Uchida A, Ishiwata S, Fang Y, Zhou X, Masuko T, Kinoshita M, Kakehi K, Sugiura R.
タイトル The cell surface protein gene ecm33+ is a target of the two transcription factors Atf1 and Mbx1 and negatively regulates Pmk1 MAPK cell integrity signaling in fission yeast.
ジャーナル Mol Biol Cell
Abstract The highly conserved fission yeast Pmk1 MAPK pathway plays a key role in cell integrity by regulating Atf1, which belongs to the ATF/cAMP-responsive element-binding (CREB) protein family. We identified and characterized ecm33(+), which encodes a glycosyl-phosphatidylinositol (GPI)-anchored cell surface protein as a transcriptional target of Pmk1 and Atf1. We demonstrated that the gene expression of Ecm33 is regulated by two transcription factors Atf1 and a MADS-box-type transcription factor Mbx1. We identified a putative ATF/CREB-binding site and an RLM1-binding site in the ecm33(+) promoter region and monitored the transcriptional activity of Atf1 or Mbx1 in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of the CRE and six tandem repeats of the Rlm1-binding sequence, respectively. These reporter genes reflect the activation of the Pmk1 pathway by various stimuli, thereby enabling the real-time monitoring of the Pmk1 cell integrity pathway. Notably, the Deltaecm33 cells displayed hyperactivation of the Pmk1 signaling together with hypersensitivity to Ca(2+) and an abnormal morphology, which were almost abolished by simultaneous deletion of the components of the Rho2/Pck2/Pmk1 pathway. Our results suggest that Ecm33 is involved in the negative feedback regulation of Pmk1 cell integrity signaling and is linked to cellular Ca(2+) signaling.
巻・号 21(4)
ページ 674-85
公開日 2010-2-15
DOI 10.1091/mbc.e09-09-0810
PII E09-09-0810
PMID 20032302
PMC PMC2820430
MeSH Activating Transcription Factor 1 / genetics Activating Transcription Factor 1 / metabolism* Calcium / metabolism Gene Expression Regulation, Fungal Genes, Reporter Glycosylphosphatidylinositols / metabolism MAP Kinase Signaling System / physiology* Membrane Proteins / genetics* Membrane Proteins / metabolism Mitogen-Activated Protein Kinases / genetics Mitogen-Activated Protein Kinases / metabolism* Promoter Regions, Genetic Schizosaccharomyces / cytology Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism*
IF 3.791
引用数 23
WOS 分野 CELL BIOLOGY
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