論文 - 詳細
| RRC ID | 11368 |
|---|---|
| 著者 | Takada H, Nishida A, Domae M, Kita A, Yamano Y, Uchida A, Ishiwata S, Fang Y, Zhou X, Masuko T, Kinoshita M, Kakehi K, Sugiura R. |
| タイトル | The cell surface protein gene ecm33+ is a target of the two transcription factors Atf1 and Mbx1 and negatively regulates Pmk1 MAPK cell integrity signaling in fission yeast. |
| ジャーナル | Mol Biol Cell |
| Abstract |
The highly conserved fission yeast Pmk1 MAPK pathway plays a key role in cell integrity by regulating Atf1, which belongs to the ATF/cAMP-responsive element-binding (CREB) protein family. We identified and characterized ecm33(+), which encodes a glycosyl-phosphatidylinositol (GPI)-anchored cell surface protein as a transcriptional target of Pmk1 and Atf1. We demonstrated that the gene expression of Ecm33 is regulated by two transcription factors Atf1 and a MADS-box-type transcription factor Mbx1. We identified a putative ATF/CREB-binding site and an RLM1-binding site in the ecm33(+) promoter region and monitored the transcriptional activity of Atf1 or Mbx1 in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of the CRE and six tandem repeats of the Rlm1-binding sequence, respectively. These reporter genes reflect the activation of the Pmk1 pathway by various stimuli, thereby enabling the real-time monitoring of the Pmk1 cell integrity pathway. Notably, the Deltaecm33 cells displayed hyperactivation of the Pmk1 signaling together with hypersensitivity to Ca(2+) and an abnormal morphology, which were almost abolished by simultaneous deletion of the components of the Rho2/Pck2/Pmk1 pathway. Our results suggest that Ecm33 is involved in the negative feedback regulation of Pmk1 cell integrity signaling and is linked to cellular Ca(2+) signaling. |
| 巻・号 | 21(4) |
| ページ | 674-85 |
| 公開日 | 2010-2-15 |
| DOI | 10.1091/mbc.e09-09-0810 |
| PII | E09-09-0810 |
| PMID | 20032302 |
| PMC | PMC2820430 |
| MeSH | Activating Transcription Factor 1 / genetics Activating Transcription Factor 1 / metabolism* Calcium / metabolism Gene Expression Regulation, Fungal Genes, Reporter Glycosylphosphatidylinositols / metabolism MAP Kinase Signaling System / physiology* Membrane Proteins / genetics* Membrane Proteins / metabolism Mitogen-Activated Protein Kinases / genetics Mitogen-Activated Protein Kinases / metabolism* Promoter Regions, Genetic Schizosaccharomyces / cytology Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* |
| IF | 3.791 |
| 引用数 | 23 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | |