論文 - 詳細
| RRC ID | 11391 |
|---|---|
| 著者 | Kataoka N, Miyake S, Azuma M. |
| タイトル | Aquaporin and aquaglyceroporin in silkworms, differently expressed in the hindgut and midgut of Bombyx mori. |
| ジャーナル | Insect Mol Biol |
| Abstract |
Two cDNAs similar to aquaporins (AQPs) from other insect species were identified and characterized from the silkworm larva, Bombyx mori. The first cDNA (AQP-Bom1) cloned from the anterior silk gland encodes a 25 900 Da protein similar to insect AQPs isolated from several liquid-feeding insects. The second cDNA (AQP-Bom2) cloned from the posterior midgut encodes a 27 694 Da protein. Northern blot analysis has revealed that the AQP-Bom1 mRNA (2.3 kb) is expressed predominantly in the hindgut (colon and rectum), and moderately or minimally in the silk gland, midgut and Malpighian tubules, while the AQP-Bom2 mRNA (1.3 kb) is mainly expressed in the posterior midgut and Malpighian tubules. Functional analysis in Xenopus oocytes microinjected with the cRNA of these AQPs revealed that the AQP-Bom1 mRNA encodes a water-specific aquaporin, likely involved in the water retrieval function of the hindgut, while the AQP-Bom2 mRNA encodes an aquaglyceroporin, increasing glycerol and urea uptake. |
| 巻・号 | 18(3) |
| ページ | 303-14 |
| 公開日 | 2009-6-1 |
| DOI | 10.1111/j.1365-2583.2009.00871.x |
| PII | IMB871 |
| PMID | 19366375 |
| MeSH | Amino Acid Sequence Animals Aquaglyceroporins / genetics Aquaglyceroporins / metabolism* Base Sequence Bombyx / genetics Bombyx / metabolism* Gastrointestinal Tract / metabolism Glycerol / metabolism Homeostasis Larva / metabolism Molecular Sequence Data Oocytes Permeability RNA, Messenger / metabolism Sequence Analysis, DNA Urea / metabolism Water / metabolism Xenopus |
| IF | 2.533 |
| 引用数 | 43 |
| WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 各媒体での言及数の合計 | 0 |
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