RRC ID 11392
Author Sugiyama T, Sugioka-Sugiyama R.
Title Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast.
Journal EMBO J.
Abstract Meiosis-specific mRNAs are transcribed in vegetative fission yeast, and these meiotic mRNAs are selectively removed from mitotic cells to suppress meiosis. This RNA elimination system requires degradation signal sequences called determinant of selective removal (DSR), an RNA-binding protein Mmi1, polyadenylation factors, and the nuclear exosome. However, the detailed mechanism by which meiotic mRNAs are selectively degraded in mitosis but not meiosis is not understood fully. Here we report that Red1, a novel protein, is essential for elimination of meiotic mRNAs from mitotic cells. A red1 deletion results in the accumulation of a large number of meiotic mRNAs in mitotic cells. Red1 interacts with Mmi1, Pla1, the canonical poly(A) polymerase, and Rrp6, a subunit of the nuclear exosome, and promotes the destabilization of DSR-containing mRNAs. Moreover, Red1 forms nuclear bodies in mitotic cells, and these foci are disassembled during meiosis. These results demonstrate that Red1 is involved in DSR-directed RNA decay to prevent ectopic expression of meiotic mRNAs in vegetative cells.
Volume 30(6)
Pages 1027-39
Published 2011-3-16
DOI 10.1038/emboj.2011.32
PII emboj201132
PMID 21317872
PMC PMC3061036
MeSH Fungal Proteins / genetics Fungal Proteins / metabolism* Gene Deletion Meiosis* Mitosis* Protein Interaction Mapping RNA Stability* RNA, Messenger / metabolism* Schizosaccharomyces / growth & development* Schizosaccharomyces / metabolism*
IF 10.557
Times Cited 33
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Yeast FY7354 FY12806 FY13293 FY13308 FY16231 FY16846