論文 - 詳細
| RRC ID | 11399 |
|---|---|
| 著者 | Simkhada D, Lee HC, Sohng JK. |
| タイトル | Genetic engineering approach for the production of rhamnosyl and allosyl flavonoids from Escherichia coli. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
The main functions of glycosylation are stabilization, detoxification and solubilization of substrates and products. To produce glycosylated products, Escherichia coli was engineered by overexpression of TDP-L-rhamnose and TDP-6-deoxy-D-allose biosynthetic gene clusters, and flavonoids were glycosylated by the overexpression of the glycosyltransferase gene from Arabidopsis thaliana. For the glycosylation, these flavonoids (quercetin and kaempferol) were exogenously fed to the host in a biotransformation system. The products were isolated, analyzed and confirmed by HPLC, LC/MS, and ESI-MS/MS analyses. Several conditions (arabinose, IPTG concentration, OD(600), substrate concentration, incubation time) were optimized to increase the production level. We successfully isolated approximately 24 mg/L 3-O-rhamnosyl quercetin and 12.9 mg/L 3-O-rhamnosyl kaempferol upon feeding of 0.2 mM of the respective flavonoids and were also able to isolate 3-O-allosyl quercetin. Thus, this study reveals a method that might be useful for the biosynthesis of rhamnosyl and allosyl flavonoids and for the glycosylation of related compounds. |
| 巻・号 | 107(1) |
| ページ | 154-62 |
| 公開日 | 2010-9-1 |
| DOI | 10.1002/bit.22782 |
| PMID | 20506539 |
| MeSH | Escherichia coli / physiology* Flavonoids / metabolism* Glycation End Products, Advanced / metabolism* Nucleoside Diphosphate Sugars / genetics Nucleoside Diphosphate Sugars / metabolism* Protein Engineering / methods* Rhamnose / analogs & derivatives* Rhamnose / genetics Rhamnose / metabolism Rutin / metabolism* Thymine Nucleotides / genetics Thymine Nucleotides / metabolism* |
| IF | 4.002 |
| 引用数 | 32 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda01903 |