RRC ID 11408
著者 Elrouby N, Coupland G.
タイトル Proteome-wide screens for small ubiquitin-like modifier (SUMO) substrates identify Arabidopsis proteins implicated in diverse biological processes.
ジャーナル Proc Natl Acad Sci U S A
Abstract Covalent modification of proteins by small ubiquitin-like modifier (SUMO) regulates various cellular activities in yeast and mammalian cells. In Arabidopsis, inactivation of genes encoding SUMO or SUMO-conjugation enzymes is lethal, emphasizing the importance of SUMOylation in plant development. Despite this, little is known about SUMO targets in plants. Here we identified 238 Arabidopsis proteins as potential SUMO substrates because they interacted with SUMO-conjugating enzyme and/or SUMO protease (ESD4) in the yeast two-hybrid system. Compared with the whole Arabidopsis proteome, the identified proteins were strongly enriched for those containing high-probability consensus SUMO attachment sites, further supporting that they are true SUMO substrates. A high-throughput assay was developed in Escherichia coli and used to test the SUMOylation of 56% of these proteins. More than 92% of the proteins tested were SUMOylated in this assay by at least one SUMO isoform. Furthermore, ADA2b, an ESD4 interactor that was SUMOylated in the E. coli system, also was shown to be SUMOylated in Arabidopsis. The identified SUMO substrates are involved in a wide range of plant processes, many of which were not previously known to involve SUMOylation. These proteins provide a basis for exploring the function of SUMOylation in the regulation of diverse processes in Arabidopsis.
巻・号 107(40)
ページ 17415-20
公開日 2010-10-5
DOI 10.1073/pnas.1005452107
PII 1005452107
PMID 20855607
PMC PMC2951436
MeSH Animals Arabidopsis / physiology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Protein Processing, Post-Translational Proteome / analysis* Small Ubiquitin-Related Modifier Proteins / genetics Small Ubiquitin-Related Modifier Proteins / metabolism* Stress, Physiological Transcription Factors / genetics Transcription Factors / metabolism Two-Hybrid System Techniques
IF 9.412
引用数 108
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda05069 pda07240 pda04156 pda06429 pda13799 pda13356 pda20993 pda10258 pda10298 pda01330 pda05051 pda00933