RRC ID 1141
Author Ito M, Deguchi T, Mizutani KS, Yasuda M, Yokoi S, Ito S, Takahashi Y, Ishihara S, Kawamura Y, Ezaki T.
Title Emergence and spread of Neisseria gonorrhoeae clinical isolates harboring mosaic-like structure of penicillin-binding protein 2 in Central Japan.
Journal Antimicrob. Agents Chemother.
Abstract Of 150 clinical isolates of Neisseria gonorrhoeae recovered in 2001, we examined 55 clinical isolates of N. gonorrhoeae for which cefixime MICs were > or=0.125 microg/ml and randomly selected 15 isolates for which cefixime MICs were < or =0.06 microg/ml for analysis of alterations in the penicillin-binding protein 2 (PBP 2) gene. We found insertion of an extra codon (Asp-345a) in the transpeptidase domain of PBP 2, and this insertion occurred alone or in conjunction with other amino acid substitutions. We also found a mosaic PBP 2 that was composed of fragments of the PBP 2 proteins from Neisseria cinera and Neisseria perflava. This mosaic PBP 2 was significantly associated with decreased susceptibilities to penicillin and cephalosporins, especially oral cephalosporins. For most of the isolates with a mosaic PBP 2, the cefixime MICs were > or =0.5 microg/ml and the cefdinir MICs were > or =1 microg/ml. Analysis of chromosomal DNA restriction patterns by pulsed-field gel electrophoresis revealed that most isolates with the mosaic PBP 2 were genetically similar. The recombination events that generated the mosaic PBP 2 would likely have contributed to the decreased sensitivities to cephalosporins. Isolates with the mosaic PBP 2 appear to threaten the efficacy of the currently recommended regimen with cefixime. The emergence of such strains may be the result of the in vivo generation of clones in which interspecies recombination occurred between the penA genes of N. gonorrhoeae and commensal Neisseria species.
Volume 49(1)
Pages 137-43
Published 2005-1
DOI 10.1128/AAC.49.1.137-143.2005
PII 49/1/137
PMID 15616287
PMC PMC538884
MeSH Amino Acid Sequence Amino Acid Substitution Anti-Bacterial Agents / pharmacology Cefixime / pharmacology Drug Resistance, Bacterial Electrophoresis, Gel, Pulsed-Field Gonorrhea / epidemiology* Gonorrhea / microbiology Gonorrhea / transmission* Humans Japan / epidemiology Male Microbial Sensitivity Tests Molecular Sequence Data Neisseria / genetics Neisseria / metabolism Neisseria gonorrhoeae / drug effects* Neisseria gonorrhoeae / genetics Neisseria gonorrhoeae / isolation & purification Neisseria gonorrhoeae / metabolism Penicillin-Binding Proteins / chemistry Penicillin-Binding Proteins / genetics* Recombination, Genetic*
IF 4.715
Times Cited 103
WOS Category PHARMACOLOGY & PHARMACY MICROBIOLOGY
Resource
Pathogenic microorganisms N. gonorrhoeae?