RRC ID 11415
著者 Ratnayake RM, Inoue H, Nonami H, Akita M.
タイトル Alternative processing of Arabidopsis Hsp70 precursors during protein import into chloroplasts.
ジャーナル Biosci Biotechnol Biochem
Abstract During protein import into chloroplasts, one of the Hsp70 proteins in pea (Hsp70-IAP), previously reported to localize in the intermembrane space of chloroplasts, was found to interact with the translocating precursor protein but the gene for Hsp70-IAP has not been identified yet. In an attempt to identify the Arabidopsis homolog of Hsp70-IAP, we employed an in vitro protein import assay to determine the localization of three Arabidopsis Hsp70 homologs (AtHsp70-6 through 8), predicted for chloroplast targeting. AtHsp70-6 and AtHsp70-7 were imported into chloroplasts and processed into similar-sized mature forms. In addition, a smaller-sized processed form of AtHsp70-6 was observed. All the processed forms of both AtHsp70 proteins were localized in the stroma. Organelle-free processing assays revealed that the larger processed forms of both AtHsp70-6 and AtHsp70-7 were cleaved by stromal processing peptidase, whereas the smaller processed form of AtHsp70-6 was produced by an unspecified peptidase.
巻・号 72(11)
ページ 2926-35
公開日 2008-11-1
DOI 10.1271/bbb.80408
PII JST.JSTAGE/bbb/80408
PMID 18997426
MeSH Amino Acid Sequence Animals Antibodies, Monoclonal / metabolism Arabidopsis / cytology* Arabidopsis / metabolism* Chloroplasts / metabolism* Computational Biology Glycine HSP70 Heat-Shock Proteins / chemistry HSP70 Heat-Shock Proteins / metabolism* Humans Mice Molecular Sequence Data Plant Proteins / chemistry Plant Proteins / metabolism* Protein Precursors / chemistry Protein Precursors / metabolism* Protein Transport Time Factors Trypsin / metabolism
IF 1.516
引用数 27
WOS 分野 FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda07010 pda09985