RRC ID 11420
Author Zhao ZG, Zhu SS, Zhang YH, Bian XF, Wang Y, Jiang L, Liu X, Chen LM, Liu SJ, Zhang WW, Ikehashi H, Wan JM.
Title Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.).
Journal Planta
Abstract Hybrid sterility hinders the exploitation of the heterosis displayed by japonica × indica rice hybrids. The variation in pollen semi-sterility observed among hybrids between the japonica recipient cultivar and each of two sets of chromosome segment substitution lines involving introgression from an indica cultivar was due to a factor on chromosome 5 known to harbor the gene S24. S24 was fine mapped to a 42 kb segment by analyzing a large F(2) population bred from the cross S24-NIL × Asominori, while the semi-sterility shown by the F(1) hybrid was ascribable to mitotic failure at the early bicellular pollen stage. Interestingly, two other pollen sterility genes (f5-Du and Sb) map to the same region (Li et al. in Chin Sci Bull 51:675-680, 2006; Wang et al. in Theor Appl Genet 112:382-387, 2006), allowing a search for candidate genes in the 6.4 kb overlap between the three genes. By sequencing the overlapped fragment in wild rice, indica cultivars and japonica cultivars, a protein ankyrin-3 encoded by the ORF2 was identified as the molecular base for S24. A cultivar Dular was found to have a hybrid-sterility-neutral allele, S24-n, in which an insertion of 30 bp was confirmed. Thus, it was possible to add one more case of molecular bases for the hybrid sterility. No gamete abortion is caused on heterozygous maternal genotype with an impaired sequence from the hybrid-sterility-neutral genotype. This result will be useful in understanding of wide compatibility in rice breeding.
Volume 233(3)
Pages 485-94
Published 2011-3
DOI 10.1007/s00425-010-1313-8
PMID 21082325
MeSH Chromosome Mapping Gene Expression Regulation, Plant Genes, Plant / genetics* Genotype Hybridization, Genetic / genetics* Mutagenesis, Insertional Oryza / genetics* Oryza / physiology Phenotype Plant Infertility / genetics* Pollen / classification Pollen / genetics* Pollen / physiology Polymorphism, Genetic / genetics
IF 3.249
Times Cited 5