論文 - 詳細
| RRC ID | 11439 |
|---|---|
| 著者 | Suehiro Y, Kinoshita M, Okuyama T, Shimada A, Naruse K, Takeda H, Kubo T, Hashimoto M, Takeuchi H. |
| タイトル | Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system. |
| ジャーナル | FEBS Lett |
| Abstract |
In this study, we demonstrated that human type-5 adenovirus infected the brain of the teleost fish, medaka (Oryzias latipes), in vivo. Injection of adenoviral vector into the mesencephalic ventricle of medaka larvae induced the expression of reporter genes in some parts of the telencephalon, the periventricular area of the mesencephalon and diencephalon, and the cerebellum. Additionally, the Cre-loxP system works in medaka brains using transgenic medaka carrying a vector containing DsRed2, flanked by loxP sites under control of the beta-actin promoter and downstream promoterless enhanced green fluorescent protein (EGFP). We demonstrated that the presence of green fluorescence depended on injection of adenoviral vector expressing the Cre gene and confirmed that EGFP mRNA was transcribed in the virus-injected larvae. |
| 巻・号 | 584(16) |
| ページ | 3545-9 |
| 公開日 | 2010-8-20 |
| DOI | 10.1016/j.febslet.2010.06.047 |
| PII | S0014-5793(10)00558-2 |
| PMID | 20621097 |
| MeSH | Adenoviruses, Human / genetics* Animals Animals, Genetically Modified Brain / anatomy & histology Brain / metabolism* Brain / virology* Gene Expression Gene Transfer Techniques* Genes, Reporter Genetic Vectors Humans Integrases Luminescent Proteins / genetics Oryzias / anatomy & histology Oryzias / genetics* Oryzias / virology* Recombinant Proteins / genetics |
| IF | 3.057 |
| 引用数 | 6 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | MT837 (d-rR/TOKYO) TG861 (d-rR-Tg (beta-actin-loxP-DsRed2-loxP-GFP)) |