論文 - 詳細
| RRC ID | 11443 |
|---|---|
| 著者 | Shibata Y, Paul-Prasanth B, Suzuki A, Usami T, Nakamoto M, Matsuda M, Nagahama Y. |
| タイトル | Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka. |
| ジャーナル | Gene Expr Patterns |
| Abstract |
In the teleost fish, medaka (Oryzias latipes), the sex is genetically determined at the time of fertilization. The males are heterogametic with XY chromosome composition, while females are of XX chromosome composition. The male sexual differentiation is initiated in XY embryos of medaka by the sex-determining gene Dmy. In this study, we have cloned the gonadal soma derived factor (Gsdf) from medaka and characterized its expression pattern during the initiation of morphological testicular differentiation. By real-time PCR, an XY-specific up-regulation was detected in the expression levels of Gsdf in the whole embryos of medaka at 6days post fertilization (dpf), coincident with the initiation of testicular differentiation in the XY gonads. Whole mount and section in situ hybridizations reaffirmed that Gsdf was expressed exclusively in primordial gonads of only the genetic males at 6dpf. Conversely, the expression of Gsdf was found to be very weak in the XX gonads during embryogenesis. Importantly, Gsdf and Dmy were found to be co-localized in the same somatic cells in the XY gonads. When the XY embryos were treated with estradiol-17beta, in order to reverse their phenotypic sex, a decline was observed in the expression of Gsdf in these embryos by real-time PCR. |
| 巻・号 | 10(6) |
| ページ | 283-9 |
| 公開日 | 2010-9-1 |
| DOI | 10.1016/j.gep.2010.06.005 |
| PII | S1567-133X(10)00065-7 |
| PMID | 20601164 |
| MeSH | Amino Acid Sequence Animals Embryo, Nonmammalian Female Fish Proteins / genetics* Fish Proteins / metabolism Gene Expression Regulation, Developmental Germ Cells / metabolism Germ Cells / physiology Gonads / embryology Gonads / metabolism Male Molecular Sequence Data Oryzias / embryology* Oryzias / genetics* Oryzias / metabolism Phylogeny Sequence Homology, Amino Acid Sex Differentiation / genetics* Testis / embryology Testis / metabolism* Time Factors |
| IF | 0.897 |
| 引用数 | 98 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | Qurt (MT828) Closed colony of HdrR-II1 (IB178) |