RRC ID 11457
Author Watanabe T, Watanabe I, Yamamoto M, Ando A, Nakamura T.
Title A UV-induced mutant of Pichia stipitis with increased ethanol production from xylose and selection of a spontaneous mutant with increased ethanol tolerance.
Journal Bioresour. Technol.
Abstract In the fermentation process of lignocellulosic biomass (such as wood and rice straw), efficient conversion of pentose (mainly xylose) into ethanol is important. Mutants of Pichia stipitis NBRC1687 were obtained after UV mutagenesis and selection of large colonies on ethanol-containing medium. One mutant, PXF58, produced 4.3% ethanol from 11.4% xylose while the parent strain only produced 3.1%. The ethanol productivities of PXF58 from glucose and fructose were about were about 1.4-fold higher than those of the parent strain. After continuous cultivation of PXF58 in YNB (yeast nitrogen base) medium containing 2% xylose and 5-7% ethanol, an ethanol-tolerant mutant, PET41, was obtained. Strain PET41 was able to produce 4.4% ethanol when first supplied with xylose then with glucose. This isolate might be thus useful for two-phase fermentation in which xylan is saccharified by xylanase to produce xylose, and glucan is saccharified later by cellulase and β-glucosidase to produce glucose.
Volume 102(2)
Pages 1844-8
Published 2011-1
DOI 10.1016/j.biortech.2010.09.087
PII S0960-8524(10)01619-6
PMID 20947339
MeSH Adaptation, Physiological* / drug effects Carbohydrates / pharmacology Ethanol / chemical synthesis* Fermentation / drug effects Fermentation / genetics Fermentation / radiation effects Mutation / genetics* Pichia / drug effects Pichia / genetics* Pichia / radiation effects* Time Factors Ultraviolet Rays* Xylose / metabolism*
IF 5.807
Times Cited 14
General Microbes JCM 9837 JCM 9842