Reference - Detail
| RRC ID | 11457 |
|---|---|
| Author | Watanabe T, Watanabe I, Yamamoto M, Ando A, Nakamura T. |
| Title | A UV-induced mutant of Pichia stipitis with increased ethanol production from xylose and selection of a spontaneous mutant with increased ethanol tolerance. |
| Journal | Bioresour Technol |
| Abstract |
In the fermentation process of lignocellulosic biomass (such as wood and rice straw), efficient conversion of pentose (mainly xylose) into ethanol is important. Mutants of Pichia stipitis NBRC1687 were obtained after UV mutagenesis and selection of large colonies on ethanol-containing medium. One mutant, PXF58, produced 4.3% ethanol from 11.4% xylose while the parent strain only produced 3.1%. The ethanol productivities of PXF58 from glucose and fructose were about were about 1.4-fold higher than those of the parent strain. After continuous cultivation of PXF58 in YNB (yeast nitrogen base) medium containing 2% xylose and 5-7% ethanol, an ethanol-tolerant mutant, PET41, was obtained. Strain PET41 was able to produce 4.4% ethanol when first supplied with xylose then with glucose. This isolate might be thus useful for two-phase fermentation in which xylan is saccharified by xylanase to produce xylose, and glucan is saccharified later by cellulase and β-glucosidase to produce glucose. |
| Volume | 102(2) |
| Pages | 1844-8 |
| Published | 2011-1-1 |
| DOI | 10.1016/j.biortech.2010.09.087 |
| PII | S0960-8524(10)01619-6 |
| PMID | 20947339 |
| MeSH | Adaptation, Physiological* / drug effects Carbohydrates / pharmacology Ethanol / chemical synthesis* Fermentation / drug effects Fermentation / genetics Fermentation / radiation effects Mutation / genetics* Pichia / drug effects Pichia / genetics* Pichia / radiation effects* Time Factors Ultraviolet Rays* Xylose / metabolism* |
| IF | 7.539 |
| Times Cited | 28 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY AGRICULTURAL ENGINEERING ENERGY & FUELS |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 9837 JCM 9842 |