RRC ID |
11457
|
著者 |
Watanabe T, Watanabe I, Yamamoto M, Ando A, Nakamura T.
|
タイトル |
A UV-induced mutant of Pichia stipitis with increased ethanol production from xylose and selection of a spontaneous mutant with increased ethanol tolerance.
|
ジャーナル |
Bioresour Technol
|
Abstract |
In the fermentation process of lignocellulosic biomass (such as wood and rice straw), efficient conversion of pentose (mainly xylose) into ethanol is important. Mutants of Pichia stipitis NBRC1687 were obtained after UV mutagenesis and selection of large colonies on ethanol-containing medium. One mutant, PXF58, produced 4.3% ethanol from 11.4% xylose while the parent strain only produced 3.1%. The ethanol productivities of PXF58 from glucose and fructose were about were about 1.4-fold higher than those of the parent strain. After continuous cultivation of PXF58 in YNB (yeast nitrogen base) medium containing 2% xylose and 5-7% ethanol, an ethanol-tolerant mutant, PET41, was obtained. Strain PET41 was able to produce 4.4% ethanol when first supplied with xylose then with glucose. This isolate might be thus useful for two-phase fermentation in which xylan is saccharified by xylanase to produce xylose, and glucan is saccharified later by cellulase and β-glucosidase to produce glucose.
|
巻・号 |
102(2)
|
ページ |
1844-8
|
公開日 |
2011-1-1
|
DOI |
10.1016/j.biortech.2010.09.087
|
PII |
S0960-8524(10)01619-6
|
PMID |
20947339
|
MeSH |
Adaptation, Physiological* / drug effects
Carbohydrates / pharmacology
Ethanol / chemical synthesis*
Fermentation / drug effects
Fermentation / genetics
Fermentation / radiation effects
Mutation / genetics*
Pichia / drug effects
Pichia / genetics*
Pichia / radiation effects*
Time Factors
Ultraviolet Rays*
Xylose / metabolism*
|
IF |
7.539
|
引用数 |
28
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
AGRICULTURAL ENGINEERING
ENERGY & FUELS
|
リソース情報 |
一般微生物 |
JCM 9837
JCM 9842 |