| RRC ID |
11467
|
| 著者 |
Saito S, Kobayashi M, Kimoto-Nira H, Aoki R, Mizumachi K, Miyata S, Yamamoto K, Kitagawa Y, Suzuki C.
|
| タイトル |
Intraspecies discrimination of Lactobacillus paraplantarum by PCR.
|
| ジャーナル |
FEMS Microbiol Lett
|
| Abstract |
Lactobacillus paraplantarum is a species phenotypically close to Lactobacillus plantarum. Several PCR methods were evaluated to discriminate L. paraplantarum strains and among them, a PCR using an enterobacterial repetitive intergenic consensus (ERIC) sequence differentiated L. paraplantarum from other Lactobacillus species. In addition, a combination of ERIC and random amplified polymorphic DNA (RAPD) analysis distinguished among seven strains of L. paraplantarum tested. ERIC-PCR profiles showed several strain-specific DNA fragments in L. paraplantarum, among them, a 2.2-kb ERIC marker, termed LpF1, found to be specific to strain FBA1, which improved the skin integrity in an animal model. The LpF1 encodes three proteins similar to Lactobacillus fermentum AroA, TyrA, and AroK, which are involved in the shikimate pathway. A primer pair specific to FBA1 based on the internal sequence of LpF1 amplified a 950-bp FBA1-specific fragment LpF2. Southern blot analysis of Dra I-digested genomic DNA of L. paraplantarum strains using LpF2 as a probe showed that LpF2 is distinctive of strain FBA1 among 16 L. paraplantarum strains. Because both ERIC- and RAPD-PCR are fast and technically simple methods, they are useful for the rapid discrimination of L. paraplantarum strains and for the development of new strain-specific DNA markers for identifying industrially important strains.
|
| 巻・号 |
316(1)
|
| ページ |
70-6
|
| 公開日 |
2011-3-1
|
| DOI |
10.1111/j.1574-6968.2010.02193.x
|
| PMID |
21204929
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Typing Techniques / methods*
DNA Primers / genetics
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Lactobacillus / classification*
Lactobacillus / genetics*
Molecular Sequence Data
Oligonucleotide Probes / genetics
Polymerase Chain Reaction / methods*
Sequence Analysis, DNA
|
| IF |
1.987
|
| 引用数 |
6
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1096
JCM 12533
JCM 1558
JCM 1149
JCM 1157 |
