Reference - Detail
| RRC ID | 11515 |
|---|---|
| Author | Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, Suganuma T. |
| Title | Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family. |
| Journal | J Biol Chem |
| Abstract |
Extensin is a glycoprotein that is rich in hydroxyprolines linked to β-L-arabinofuranosides. In this study, we cloned a hypBA2 gene that encodes a novel β-L-arabinobiosidase from Bifidobacterium longum JCM 1217. This enzyme does not have any sequence similarity with other glycoside hydrolase families but has 38-98% identity to hypothetical proteins in Bifidobacterium and Xanthomonas strains. The recombinant enzyme liberated L-arabinofuranose (Araf)-β1,2-Araf disaccharide from carrot extensin, potato lectin, and Araf-β1,2-Araf-β1,2-Araf-β-Hyp (Ara(3)-Hyp) but not Araf-α1,3-Araf-β1,2-Araf-β1,2-Araf-β-Hyp (Ara(4)-Hyp) or Araf-β1,2-Araf-β-Hyp (Ara(2)-Hyp), which indicated that it was specific for unmodified Ara(3)-Hyp substrate. The enzyme also transglycosylated 1-alkanols with retention of the anomeric configuration. This is the first report of an enzyme that hydrolyzes Hyp-linked β-L-arabinofuranosides, which defines a new family of glycoside hydrolases, glycoside hydrolase family 121. |
| Volume | 286(7) |
| Pages | 5143-50 |
| Published | 2011-2-18 |
| DOI | 10.1074/jbc.M110.190512 |
| PII | S0021-9258(20)52083-8 |
| PMID | 21149454 |
| PMC | PMC3037626 |
| MeSH | Amino Acid Sequence Arabinose / analogs & derivatives Arabinose / chemistry Arabinose / metabolism Bacterial Proteins / chemistry* Bacterial Proteins / genetics Bacterial Proteins / metabolism Bifidobacterium / enzymology* Bifidobacterium / genetics Cloning, Molecular Glycoside Hydrolases / chemistry* Glycoside Hydrolases / genetics Glycoside Hydrolases / metabolism Molecular Sequence Data Xanthomonas / enzymology Xanthomonas / genetics |
| IF | 4.238 |
| Times Cited | 30 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| General Microbes | JCM 1217 JCM 7054 JCM 1275 JCM 1254 JCM 1192 JCM 1222 JCM 1205 |