論文 - 詳細
RRC ID | 11515 |
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著者 | Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, Suganuma T. |
タイトル | Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family. |
ジャーナル | J Biol Chem |
Abstract |
Extensin is a glycoprotein that is rich in hydroxyprolines linked to β-L-arabinofuranosides. In this study, we cloned a hypBA2 gene that encodes a novel β-L-arabinobiosidase from Bifidobacterium longum JCM 1217. This enzyme does not have any sequence similarity with other glycoside hydrolase families but has 38-98% identity to hypothetical proteins in Bifidobacterium and Xanthomonas strains. The recombinant enzyme liberated L-arabinofuranose (Araf)-β1,2-Araf disaccharide from carrot extensin, potato lectin, and Araf-β1,2-Araf-β1,2-Araf-β-Hyp (Ara(3)-Hyp) but not Araf-α1,3-Araf-β1,2-Araf-β1,2-Araf-β-Hyp (Ara(4)-Hyp) or Araf-β1,2-Araf-β-Hyp (Ara(2)-Hyp), which indicated that it was specific for unmodified Ara(3)-Hyp substrate. The enzyme also transglycosylated 1-alkanols with retention of the anomeric configuration. This is the first report of an enzyme that hydrolyzes Hyp-linked β-L-arabinofuranosides, which defines a new family of glycoside hydrolases, glycoside hydrolase family 121. |
巻・号 | 286(7) |
ページ | 5143-50 |
公開日 | 2011-2-18 |
DOI | 10.1074/jbc.M110.190512 |
PII | S0021-9258(20)52083-8 |
PMID | 21149454 |
PMC | PMC3037626 |
MeSH | Amino Acid Sequence Arabinose / analogs & derivatives Arabinose / chemistry Arabinose / metabolism Bacterial Proteins / chemistry* Bacterial Proteins / genetics Bacterial Proteins / metabolism Bifidobacterium / enzymology* Bifidobacterium / genetics Cloning, Molecular Glycoside Hydrolases / chemistry* Glycoside Hydrolases / genetics Glycoside Hydrolases / metabolism Molecular Sequence Data Xanthomonas / enzymology Xanthomonas / genetics |
IF | 4.238 |
引用数 | 30 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
一般微生物 | JCM 1217 JCM 7054 JCM 1275 JCM 1254 JCM 1192 JCM 1222 JCM 1205 |