| RRC ID |
11553
|
| 著者 |
Namiki T, Niwa R, Sakudoh T, Shirai K, Takeuchi H, Kataoka H.
|
| タイトル |
Cytochrome P450 CYP307A1/Spook: a regulator for ecdysone synthesis in insects.
|
| ジャーナル |
Biochem Biophys Res Commun
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| Abstract |
The prothoracic gland (PG) has essential roles in synthesizing and secreting a steroid hormone called ecdysone that is critical for molting and metamorphosis of insects. However, little is known about the genes controlling ecdysteroidogenesis in the PG. To identify genes functioning in the PG of the silkworm, Bombyx mori, we used differential display PCR and focused on a cytochrome P450 gene designated Cyp307a1. Its expression level positively correlates with a change in the hemolymph ecdysteroid titer. In addition, Drosophila Cyp307a1 is encoded in the spook locus, one of the Halloween mutant family members showing a low ecdysone titer in vivo, suggesting that Cyp307a1 is involved in ecdysone synthesis. While Drosophila Cyp307a1 is expressed in the early embryos and adult ovaries, the expression is not observed in the PGs of embryos or third instar larvae. These results suggest a difference in the ecdysone synthesis pathways during larval development in these insects.
|
| 巻・号 |
337(1)
|
| ページ |
367-74
|
| 公開日 |
2005-11-11
|
| DOI |
10.1016/j.bbrc.2005.09.043
|
| PII |
S0006-291X(05)02005-X
|
| PMID |
16188237
|
| MeSH |
Amino Acid Sequence
Animals
Bombyx / enzymology*
Bombyx / genetics
Bombyx / growth & development
Cytochrome P-450 Enzyme System / classification
Cytochrome P-450 Enzyme System / genetics*
Cytochrome P-450 Enzyme System / metabolism*
Drosophila Proteins / genetics
Ecdysone / biosynthesis*
Gene Expression Profiling
In Situ Hybridization
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Phylogeny
RNA, Messenger / analysis
Sequence Alignment
|
| IF |
2.985
|
| 引用数 |
102
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
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| リソース情報 |
| ショウジョウバエ |
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