| RRC ID |
1155
|
| Author |
Matsumura K, Miki T, Jhomori T, Gonoi T, Seino S.
|
| Title |
Possible role of PEPT1 in gastrointestinal hormone secretion.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Oligopeptides originating from ingested meal stimulate the secretion of various gastrointestinal hormones, but the mechanism is unknown. In this study, we show that transfection of oligopeptide transporter 1 (PEPT1) in STC-1 cells, a murine enteroendocrine cell line, evokes di-peptide-stimulated hormone secretion in a pH-dependent manner. Measurement of membrane potentials shows that PEPT1- transfected STC-1 cells are depolarized by di-peptide glycyl-glycine but not by glycine monomer. Glycyl-glycine stimulation induces a rise in the intracellular calcium concentration in PEPT1-transfected STC-1 cells. The secretion induced by glycyl-glycine in PEPT1-transfected STC-1 cells was blocked by nifedipine, a Ca(2+) channel blocker, suggesting that the secretion is triggered by Ca(2+) influx through L-type voltage-dependent Ca(2+) channels. These data suggest that PEPT1 mediates oligopeptide-induced hormone secretion in enteroendocrine cells.
|
| Volume |
336(4)
|
| Pages |
1028-32
|
| Published |
2005-11-4
|
| DOI |
10.1016/j.bbrc.2005.08.259
|
| PII |
S0006-291X(05)01991-1
|
| PMID |
16181611
|
| MeSH |
Animals
Calcium / metabolism
Calcium Channel Blockers / pharmacology
Calcium Channels, L-Type / physiology
Cell Line
Enteroendocrine Cells / drug effects
Enteroendocrine Cells / physiology*
Glycine / pharmacology
Glycylglycine / pharmacology
Human Growth Hormone / genetics
Human Growth Hormone / metabolism*
Humans
Hydrogen-Ion Concentration
Intracellular Fluid / metabolism
Membrane Potentials
Mice
Nifedipine / pharmacology
Peptide Transporter 1
Recombinant Proteins / metabolism
Symporters / metabolism*
Transcription, Genetic
|
| IF |
2.985
|
| Times Cited |
37
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Pathogenic microorganisms |
|
