| RRC ID |
11564
|
| 著者 |
Takeo S, Akiyama T, Firkus C, Aigaki T, Nakato H.
|
| タイトル |
Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog.
|
| ジャーナル |
Dev Biol
|
| Abstract |
Glypicans, a family of heparan sulfate proteoglycans attached to the cell surface via a glycosylphosphatidylinositol (GPI)-anchor, play essential roles in morphogen signaling and distributions. A Drosophila glypican, Dally, regulates the gradient formation of Decapentaplegic (Dpp) in the developing wing. To gain insights into the function of glypicans in morphogen signaling, we examined the activities of two mutant forms of Dally: a transmembrane form (TM-Dally) and a secreted form (Sec-Dally). Misexpression of tm-dally in the wing disc had a similar yet weaker effect in enhancing Dpp signaling compared to that of wild-type dally. In contrast, Sec-Dally shows a weak dominant negative activity on Dpp signal transduction. Furthermore, sec-dally expression led to patterning defects as well as a substantial overgrowth of tissues and animals through the expansion of the action range of Hh. These findings support the recently proposed model that secreted glypicans have opposing and/or distinct effects on morphogen signaling from the membrane-tethered forms.
|
| 巻・号 |
284(1)
|
| ページ |
204-18
|
| 公開日 |
2005-8-1
|
| DOI |
10.1016/j.ydbio.2005.05.014
|
| PII |
S0012-1606(05)00333-7
|
| PMID |
15963974
|
| MeSH |
Animals
Body Patterning / physiology*
DNA Primers
Drosophila
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Gene Expression Regulation, Developmental*
Hedgehog Proteins
Immunoblotting
Immunohistochemistry
Membrane Glycoproteins / genetics
Membrane Glycoproteins / metabolism*
Proteoglycans / genetics
Proteoglycans / metabolism*
Signal Transduction / physiology*
Wings, Animal / embryology*
|
| IF |
2.896
|
| 引用数 |
49
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
|
