RRC ID 11569
著者 Inoshita T, Tanimura T.
タイトル Cellular identification of water gustatory receptor neurons and their central projection pattern in Drosophila.
ジャーナル Proc Natl Acad Sci U S A
Abstract Water perception is important for insects, because they are particularly vulnerable to water loss because their body size is small. In Drosophila, gustatory receptor neurons are located at the base of the taste sensilla on the labellum, tarsi, and wing margins. One of the gustatory receptor neurons in typical sensilla is known to respond to water. To reveal the neural mechanisms of water perception in Drosophila, it is necessary to identify water receptor neurons and their projection patterns. We used a Gal4 enhancer trap strain in which GAL4 is expressed in a single gustatory receptor neuron in each sensillum on the labellum. We investigated the function of these neurons by expressing the upstream activating sequence transgenes, shibire(ts1), tetanus toxin light chain, or diphtheria toxin A chain. Results from the proboscis extension reflex test and electrophysiological recordings indicated that the GAL4-expressing neurons respond to water. We show here that the water receptor neurons project to a specific region in the subesophageal ganglion, thus revealing the water taste sensory map in Drosophila.
巻・号 103(4)
ページ 1094-9
公開日 2006-1-24
DOI 10.1073/pnas.0502376103
PII 0502376103
PMID 16415164
PMC PMC1347963
MeSH Animals Brain / metabolism Cell Nucleus / metabolism Chemoreceptor Cells Drosophila Drosophila Proteins Electrophysiology Enhancer Elements, Genetic Female Genes, Insect Green Fluorescent Proteins / metabolism Male Nerve Tissue / metabolism Neurons / metabolism* Neurons, Afferent / chemistry Peripheral Nervous System / metabolism Sense Organs Shaker Superfamily of Potassium Channels / chemistry Taste Taste Buds / metabolism* Time Factors Transgenes Water / metabolism
IF 9.412
引用数 48
WOS 分野 MULTIDISCIPLINARY SCIENCES
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