RRC ID 11578
Author Shimanouchi K, Takata K, Yamaguchi M, Murakami S, Ishikawa G, Takeuchi R, Kanai Y, Ruike T, Nakamura R, Abe Y, Sakaguchi K.
Title Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells.
Journal J Biochem
Abstract The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells.
Volume 139(1)
Pages 51-8
Published 2006-1-1
DOI 10.1093/jb/mvj006
PII 139/1/51
PMID 16428319
MeSH Animals Animals, Genetically Modified DNA Damage DNA-Binding Proteins / physiology* Drosophila Female Genomic Instability* Male Wings, Animal / growth & development
IF 2.476
Times Cited 6
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Drosophila DGRC#109605