Reference - Detail
| RRC ID | 11578 |
|---|---|
| Author | Shimanouchi K, Takata K, Yamaguchi M, Murakami S, Ishikawa G, Takeuchi R, Kanai Y, Ruike T, Nakamura R, Abe Y, Sakaguchi K. |
| Title | Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. |
| Journal | J Biochem |
| Abstract |
The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells. |
| Volume | 139(1) |
| Pages | 51-8 |
| Published | 2006-1-1 |
| DOI | 10.1093/jb/mvj006 |
| PII | 139/1/51 |
| PMID | 16428319 |
| MeSH | Animals Animals, Genetically Modified DNA Damage DNA-Binding Proteins / physiology* Drosophila Female Genomic Instability* Male Wings, Animal / growth & development |
| IF | 2.476 |
| Times Cited | 6 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#109605 |