RRC ID |
11578
|
Author |
Shimanouchi K, Takata K, Yamaguchi M, Murakami S, Ishikawa G, Takeuchi R, Kanai Y, Ruike T, Nakamura R, Abe Y, Sakaguchi K.
|
Title |
Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells.
|
Journal |
J Biochem
|
Abstract |
The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells.
|
Volume |
139(1)
|
Pages |
51-8
|
Published |
2006-1-1
|
DOI |
10.1093/jb/mvj006
|
PII |
139/1/51
|
PMID |
16428319
|
MeSH |
Animals
Animals, Genetically Modified
DNA Damage
DNA-Binding Proteins / physiology*
Drosophila
Female
Genomic Instability*
Male
Wings, Animal / growth & development
|
IF |
2.476
|
Times Cited |
6
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Drosophila |
DGRC#109605 |