Reference - Detail
| RRC ID | 11595 |
|---|---|
| Author | Kohsaka H, Takasu E, Nose A. |
| Title | In vivo induction of postsynaptic molecular assembly by the cell adhesion molecule Fasciclin2. |
| Journal | J Cell Biol |
| Abstract |
Cell adhesion molecules (CAMs) are thought to mediate interactions between innervating axons and their targets. However, such interactions have not been directly observed in vivo. In this paper, we study the function and dynamics of Fasciclin2 (Fas2), a homophilic CAM expressed both pre- and postsynaptically during neuromuscular synapse formation in Drosophila melanogaster. We apply live imaging of functional fluorescent fusion proteins expressed in muscles and find that Fas2 and Discs-Large (Dlg; a scaffolding protein known to bind Fas2) accumulate at the synaptic contact site soon after the arrival of the nerve. Genetic, deletion, and photobleaching analyses suggest that Fas2-mediated trans-synaptic adhesion is important for the postsynaptic accumulation of both Fas2 itself and Dlg. In fas2 mutants, many aspects of synapse formation appear normal; however, we see a reduction in the synaptic accumulation of Scribble (another scaffolding protein) and glutamate receptor subunits GluRIIA and GluRIIB. We propose that Fas2 mediates trans-synaptic adhesion, which contributes to postsynaptic molecular assembly at the onset of synaptogenesis. |
| Volume | 179(6) |
| Pages | 1289-300 |
| Published | 2007-12-17 |
| DOI | 10.1083/jcb.200705154 |
| PII | jcb.200705154 |
| PMID | 18070911 |
| PMC | PMC2140041 |
| MeSH | Animals Cell Adhesion Molecules, Neuronal / analysis Cell Adhesion Molecules, Neuronal / metabolism Cell Adhesion Molecules, Neuronal / physiology* Drosophila Proteins / analysis Drosophila Proteins / metabolism Drosophila Proteins / physiology* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Fluorescence Recovery After Photobleaching Luminescent Proteins / analysis Models, Biological Protein Subunits / metabolism Receptors, Glutamate / metabolism Recombinant Fusion Proteins / analysis Synapses / metabolism* Tumor Suppressor Proteins / metabolism |
| IF | 8.811 |
| Times Cited | 37 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | |