論文 - 詳細
| RRC ID | 11596 |
|---|---|
| 著者 | Lancaster OM, Cullen CF, Ohkura H. |
| タイトル | NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus. |
| ジャーナル | J Cell Biol |
| Abstract |
Accurate chromosome segregation in meiosis requires dynamic changes in chromatin organization. In Drosophila melanogaster, upon completion of recombination, meiotic chromosomes form a single, compact cluster called the karyosome in an enlarged oocyte nucleus. This clustering is also found in humans; however, the mechanisms underlying karyosome formation are not understood. In this study, we report that phosphorylation of barrier to autointegration factor (BAF) by the conserved kinase nucleosomal histone kinase-1 (NHK-1; Drosophila Vrk1) has a critical function in karyosome formation. We find that the noncatalytic domain of NHK-1 is crucial for its kinase activity toward BAF, a protein that acts as a linker between chromatin and the nuclear envelope. A reduction of NHK-1 or expression of nonphosphorylatable BAF results in ectopic association of chromosomes with the nuclear envelope in oocytes. We propose that BAF phosphorylation by NHK-1 disrupts anchorage of chromosomes to the nuclear envelope, allowing karyosome formation in oocytes. These data provide the first mechanistic insight into how the karyosome forms. |
| 巻・号 | 179(5) |
| ページ | 817-24 |
| 公開日 | 2007-12-3 |
| DOI | 10.1083/jcb.200706067 |
| PII | jcb.200706067 |
| PMID | 18039935 |
| PMC | PMC2099182 |
| MeSH | Amino Acid Sequence Animals Catalytic Domain Chromatin / metabolism Chromosomes / enzymology* DNA-Binding Proteins / chemistry DNA-Binding Proteins / metabolism* Drosophila Proteins / chemistry Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / enzymology* Meiosis Models, Biological Molecular Sequence Data Mutation / genetics Nuclear Envelope / metabolism Nuclear Proteins / chemistry Nuclear Proteins / metabolism* Oocytes / cytology* Oocytes / enzymology* Phosphorylation Protamine Kinase / chemistry Protamine Kinase / metabolism* Substrate Specificity |
| IF | 8.811 |
| 引用数 | 53 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |