RRC ID 11607
著者 Tsuda M, Sugiura T, Ishii T, Ishii N, Aigaki T.
タイトル A mev-1-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila.
ジャーナル Biochem Biophys Res Commun
Abstract Oxidative stress is thought to promote aging by damaging various cellular components. We investigated the phenotypic effects of mutation in the sdhC gene encoding succinate dehydrogenase C in Drosophila by using transgenic flies expressing a dominant-negative form, SdhC(I71E). Expression of SdhC(I71E) significantly reduced the mean lifespan by 22% compared to that of control flies. The amount of protein carbonyl was significantly increased, suggesting that a high level of oxidative stress was induced in these flies. The transgenic flies should serve as a model for studying the biological processes associated with increased levels of oxidative stress and mitochondrial dysfunction in Drosophila.
巻・号 363(2)
ページ 342-6
公開日 2007-11-16
DOI 10.1016/j.bbrc.2007.08.168
PII S0006-291X(07)01899-2
PMID 17854771
MeSH Animals Bacterial Proteins / genetics Bacterial Proteins / metabolism* Caenorhabditis elegans Proteins / metabolism* Cytochromes b Drosophila / physiology* Longevity / physiology* Oxidative Stress / physiology* Succinate Dehydrogenase / metabolism*
IF 2.985
引用数 16
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
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