| RRC ID |
1160
|
| 著者 |
Biswas SK, Wang L, Yokoyama K, Nishimura K.
|
| タイトル |
Molecular phylogenetics of the genus trichosporon inferred from mitochondrial cytochrome B gene sequences.
|
| ジャーナル |
J Clin Microbiol
|
| Abstract |
Mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus Trichosporon were partially sequenced to determine their molecular phylogenetic relationships. Almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. Analysis of a 396-bp coding sequence from each strain of Trichosporon under investigation showed a total of 141 (35.6%) variable nucleotide sites. A phylogenetic tree based on the cyt b gene sequences revealed that all species of Trichosporon except Trichosporon domesticum and Trichosporon montevideense had species-specific cyt b genes. Trichosporon sp. strain CBS 5581 was identified as Trichosporon pullulans, and one clinical isolate, IFM 48794, was identified as Trichosporon faecale. Analysis of 132-bp deduced amino acid sequences showed a total of 34 (25.75%) variable amino acid sites. T. domesticum and T. montevideense, Trichosporon asahii and Trichosporon asteroides, and Trichosporon gracile and Trichosporon guehoae had identical amino acid sequences. A phylogenetic tree constructed with the ascomycetes Saccharomyces douglasii and Candida glabrata taken as outgroup species and including representative species from closely related genera species of Trichosporon clustered with other basidiomycetous yeasts that contain xylose in their cell wall compositions. These results indicate the effectiveness of mitochondrial cyt b gene sequences for both species identification and the phylogenetic analysis of Trichosporon species.
|
| 巻・号 |
43(10)
|
| ページ |
5171-8
|
| 公開日 |
2005-10-1
|
| DOI |
10.1128/JCM.43.10.5171-5178.2005
|
| PII |
43/10/5171
|
| PMID |
16207980
|
| PMC |
PMC1248456
|
| MeSH |
Amino Acid Sequence
Cytochromes b / chemistry
Cytochromes b / genetics*
DNA, Fungal / analysis
Mitochondria / enzymology*
Molecular Sequence Data
Mycological Typing Techniques
Phylogeny*
Polymerase Chain Reaction
Sequence Analysis, DNA
Species Specificity
Trichosporon / classification*
Trichosporon / genetics*
|
| IF |
5.897
|
| 引用数 |
18
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 5788
IFM 5791
IFM 45920
IFM 48186
IFM 48191
IFM 48200
IFM 48391
IFM 48429
IFM 48537
IFM 48551
IFM 48554
IFM 48555
IFM 48558
IFM 48559
IFM 48560
IFM 48561
IFM 48562
IFM 48563
IFM 48564
IFM 48565
IFM 48573
IFM 48574
IFM 48575
IFM 48576
IFM 48577
IFM 48578
IFM 48579
IFM 48580
IFM 48581
IFM 48582
IFM 48585
IFM 48608
IFM 48609
IFM 48610
IFM 48611
IFM 48612
IFM 48613
IFM 48614
IFM 48615
IFM 48622
IFM 48623
IFM 48637
IFM 48656
IFM 48657
IFM 48667
IFM 48669
IFM 48672
IFM 48675
IFM 48794
IFM 48959
IFM 48960
IFM 48961
IFM 48962
IFM 48963
IFM 49111
IFM 49208
IFM 49220
IFM 49221
IFM 49222
IFM 49223
IFM 49226
IFM 50426
IFM 50454
IFM 50466
IFM 50456 |
