RRC ID 11619
Author Ly CV, Yao CK, Verstreken P, Ohyama T, Bellen HJ.
Title straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel alpha1 subunit.
Journal J. Cell Biol.
Abstract In a screen to identify genes involved in synaptic function, we isolated mutations in Drosophila melanogaster straightjacket (stj), an alpha(2)delta subunit of the voltage-gated calcium channel. stj mutant photoreceptors develop normal synaptic connections but display reduced "on-off" transients in electroretinogram recordings, indicating a failure to evoke postsynaptic responses and, thus, a defect in neurotransmission. stj is expressed in neurons but excluded from glia. Mutants exhibit endogenous seizure-like activity, indicating altered neuronal excitability. However, at the synaptic level, stj larval neuromuscular junctions exhibit approximately fourfold reduction in synaptic release compared with controls stemming from a reduced release probability at these synapses. These defects likely stem from destabilization of Cacophony (Cac), the primary presynaptic alpha(1) subunit in D. melanogaster. Interestingly, neuronal overexpression of cac partially rescues the viability and physiological defects in stj mutants, indicating a role for the alpha(2)delta Ca(2+) channel subunit in mediating the proper localization of an alpha(1) subunit at synapses.
Volume 181(1)
Pages 157-70
Published 2008-4-7
DOI 10.1083/jcb.200712152
PII jcb.200712152
PMID 18391075
PMC PMC2287295
MeSH Animals Calcium Channels / genetics Calcium Channels / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Electroretinography Mutation Neurons / metabolism* Neurotransmitter Agents / metabolism Synapses*
IF 8.784
Times Cited 38
Drosophila NP1574-GAL4 (DGRC#104034 or DGRC#112708)