Reference - Detail
|Author||Nakamura R, Takeuchi R, Takata K, Shimanouchi K, Abe Y, Kanai Y, Ruike T, Ihara A, Sakaguchi K.|
|Title||TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster.|
|Journal||Mol Cell Biol|
The Saccharomyces cerevisiae poly(A) polymerases Trf4 and Trf5 are involved in an RNA quality control mechanism, where polyadenylated RNAs are degraded by the nuclear exosome. Although Trf4/5 homologue genes are distributed throughout multicellular organisms, their biological roles remain to be elucidated. We isolated here the two homologues of Trf4/5 in Drosophila melanogaster, named DmTRF4-1 and DmTRF4-2, and investigated their biological function. DmTRF4-1 displayed poly(A) polymerase activity in vitro, whereas DmTRF4-2 did not. Gene knockdown of DmTRF4-1 by RNA interference is lethal in flies, as is the case for the trf4 trf5 double mutants. In contrast, disruption of DmTRF4-2 results in viable flies. Cellular localization analysis suggested that DmTRF4-1 localizes in the nucleolus. Abnormal polyadenylation of snRNAs was observed in transgenic flies overexpressing DmTRF4-1 and was slightly increased by the suppression of DmRrp6, the 3'-5' exonuclease of the nuclear exosome. These results suggest that DmTRF4-1 and DmRrp6 are involved in the polyadenylation-mediated degradation of snRNAs in vivo.
|MeSH||Amino Acid Sequence Animals Cell Nucleolus / enzymology DNA-Directed DNA Polymerase / chemistry DNA-Directed DNA Polymerase / metabolism Drosophila Proteins / chemistry Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / embryology Drosophila melanogaster / enzymology* Drosophila melanogaster / genetics Genes, Developmental Genome, Insect / genetics Molecular Sequence Data Morphogenesis Mutation / genetics Polyadenylation* Polynucleotide Adenylyltransferase / chemistry Polynucleotide Adenylyltransferase / metabolism* Protein Transport RNA, Small Nuclear / metabolism* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / metabolism Sequence Homology, Nucleic Acid Subcellular Fractions / enzymology|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|
|Drosophila||CG17462[NP2505] (DGRC#104223) DGRC#109790|