RRC ID 11633
Author Ambrose RL, Lander GC, Maaty WS, Bothner B, Johnson JE, Johnson KN.
Title Drosophila A virus is an unusual RNA virus with a T=3 icosahedral core and permuted RNA-dependent RNA polymerase.
Journal J. Gen. Virol.
Abstract The vinegar fly, Drosophila melanogaster, is a popular model for the study of invertebrate antiviral immune responses. Several picorna-like viruses are commonly found in both wild and laboratory populations of D. melanogaster. The best-studied and most pathogenic of these is the dicistrovirus Drosophila C virus. Among the uncharacterized small RNA viruses of D. melanogaster, Drosophila A virus (DAV) is the least pathogenic. Historically, DAV has been labelled as a picorna-like virus based on its particle size and the content of its RNA genome. Here, we describe the characterization of both the genome and the virion structure of DAV. Unexpectedly, the DAV genome was shown to encode a circular permutation in the palm-domain motifs of the RNA-dependent RNA polymerase. This arrangement has only been described previously for a subset of viruses from the double-stranded RNA virus family Birnaviridae and the T=4 single-stranded RNA virus family Tetraviridae. The 8 A (0.8 nm) DAV virion structure computed from cryo-electron microscopy and image reconstruction indicates that the virus structural protein forms two discrete domains within the capsid. The inner domain is formed from a clear T=3 lattice with similarity to the beta-sandwich domain of tomato bushy stunt virus, whilst the outer domain is not ordered icosahedrally, but forms a cage-like structure that surrounds the core domain. Taken together, this indicates that DAV is highly divergent from previously described viruses.
Volume 90(Pt 9)
Pages 2191-200
Published 2009-9
DOI 10.1099/vir.0.012104-0
PII vir.0.012104-0
PMID 19474243
PMC PMC2742409
MeSH Amino Acid Sequence Animals Capsid Proteins / chemistry Capsid Proteins / genetics Capsid Proteins / metabolism Drosophila melanogaster / virology Molecular Sequence Data Nucleocapsid / chemistry* Nucleocapsid / genetics Nucleocapsid / metabolism Protein Structure, Tertiary RNA Replicase / genetics RNA Replicase / metabolism* RNA Viruses / chemistry* RNA Viruses / enzymology RNA Viruses / genetics RNA Viruses / physiology Viral Proteins / genetics Viral Proteins / metabolism* Virus Replication
IF 2.514
Times Cited 16
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY VIROLOGY
Resource
Drosophila Champetieres (DGRC#103403)