| RRC ID |
11651
|
| 著者 |
Carmon A, Guertin MJ, Grushko O, Marshall B, MacIntyre R.
|
| タイトル |
A molecular analysis of mutations at the complex dumpy locus in Drosophila melanogaster.
|
| ジャーナル |
PLoS One
|
| Abstract |
The Drosophila dumpy gene consists of seventy eight coding exons and encodes a huge extracellular matrix protein containing large numbers of epidermal growth factor-like (EGF) modules and a novel module called dumpy (DPY). A molecular analysis of forty five mutations in the dumpy gene of Drosophila melanogaster was carried out. Mutations in this gene affect three phenotypes: wing shape, thoracic cuticular defects, and lethality. Most of the mutations were chemically induced in a single dumpy allele and were analyzed using a nuclease that cleaves single base pair mismatches in reannealed duplexes followed by dHPLC. Additionally, several spontaneous mutations were analyzed. Virtually all of the chemically induced mutations, except for several in a single exon, either generate nonsense codons or lesions that result in downstream stop codons in the reading frame. The remaining chemically induced mutations remove splice sites in the nascent dumpy message. We propose that the vast majority of nonsense mutations that affect all three basic dumpy phenotypes are in constitutive exons, whereas nonsense mutants that remove only one or two of the basic functions are in alternatively spliced exons. Evolutionary comparisons of the dumpy gene from seven Drosophila species show strong conservation of the 5' ends of exons where mutants with partial dumpy function are found. In addition, reverse transcription PCR analyses reveal transcripts in which exons marked by nonsense mutations with partial dumpy function are absent.
|
| 巻・号 |
5(8)
|
| ページ |
e12319
|
| 公開日 |
2010-8-23
|
| DOI |
10.1371/journal.pone.0012319
|
| PMID |
20811586
|
| PMC |
PMC2930355
|
| MeSH |
Alleles
Alternative Splicing
Animals
Drosophila Proteins / genetics*
Drosophila melanogaster / genetics*
Evolution, Molecular
Extracellular Matrix Proteins / genetics*
Female
Genetic Loci / genetics*
Humans
Male
Mice
Mutation* / drug effects
Organ Specificity
Protein Isoforms / genetics
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
2.74
|
| 引用数 |
3
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| ショウジョウバエ |
dp[lDG82] dp[lDG83] |
