| RRC ID |
11654
|
| 著者 |
Cook RK, Deal ME, Deal JA, Garton RD, Brown CA, Ward ME, Andrade RS, Spana EP, Kaufman TC, Cook KR.
|
| タイトル |
A new resource for characterizing X-linked genes in Drosophila melanogaster: systematic coverage and subdivision of the X chromosome with nested, Y-linked duplications.
|
| ジャーナル |
Genetics
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| Abstract |
Interchromosomal duplications are especially important for the study of X-linked genes. Males inheriting a mutation in a vital X-linked gene cannot survive unless there is a wild-type copy of the gene duplicated elsewhere in the genome. Rescuing the lethality of an X-linked mutation with a duplication allows the mutation to be used experimentally in complementation tests and other genetic crosses and it maps the mutated gene to a defined chromosomal region. Duplications can also be used to screen for dosage-dependent enhancers and suppressors of mutant phenotypes as a way to identify genes involved in the same biological process. We describe an ongoing project in Drosophila melanogaster to generate comprehensive coverage and extensive breakpoint subdivision of the X chromosome with megabase-scale X segments borne on Y chromosomes. The in vivo method involves the creation of X inversions on attached-XY chromosomes by FLP-FRT site-specific recombination technology followed by irradiation to induce large internal X deletions. The resulting chromosomes consist of the X tip, a medial X segment placed near the tip by an inversion, and a full Y. A nested set of medial duplicated segments is derived from each inversion precursor. We have constructed a set of inversions on attached-XY chromosomes that enable us to isolate nested duplicated segments from all X regions. To date, our screens have provided a minimum of 78% X coverage with duplication breakpoints spaced a median of nine genes apart. These duplication chromosomes will be valuable resources for rescuing and mapping X-linked mutations and identifying dosage-dependent modifiers of mutant phenotypes.
|
| 巻・号 |
186(4)
|
| ページ |
1095-109
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| 公開日 |
2010-12-1
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| DOI |
10.1534/genetics.110.123265
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| PII |
genetics.110.123265
|
| PMID |
20876560
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| PMC |
PMC2998296
|
| MeSH |
Animals
Biomedical Technology / methods*
Drosophila melanogaster / genetics*
Gene Duplication*
Genes, X-Linked / genetics*
Male
Methods
Mutation
Recombination, Genetic / genetics
X Chromosome / genetics*
Y Chromosome / genetics*
|
| IF |
4.015
|
| 引用数 |
27
|
|
WOS 分野
|
GENETICS & HEREDITY
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| リソース情報 |
| ショウジョウバエ |
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