RRC ID 11659
Author Haruta T, Warrior R, Yonemura S, Oda H.
Title The proximal half of the Drosophila E-cadherin extracellular region is dispensable for many cadherin-dependent events but required for ventral furrow formation.
Journal Genes Cells
Abstract The formation of the ventral furrow during Drosophila gastrulation is driven by coordinated apical constriction. Cell-cell adhesion is thought to regulate apical constriction, but the mechanisms are poorly understood. DE-cadherin, an epithelial classic cadherin, has in its membrane-proximal extracellular region a suite of domains absent from vertebrate/urochordate classic cadherins. We constructed DEΔP, a DE-cadherin derivative that lacks the membrane-proximal half of the extracellular region but retains the entire cytoplasmic domain and still exhibits strong cell-cell binding ability. The extracellular region of DEΔP consists of only cadherin repeats, mimicking vertebrate/urochordate classic cadherins. In animals lacking DE-cadherin, DEΔP organized adherens junction assembly and functioned fully in many cadherin-dependent processes, including oogenesis. Embryos in which DE-cadherin was entirely replaced by DEΔP established the blastoderm epithelium but failed to form a ventral furrow. Apical constrictions were initiated relatively normally but subsequently decelerated. These were then followed by catastrophic disruption of the junctional network. Our results suggest that although the membrane-proximal half of the DE-cadherin extracellular region is dispensable for many developmental events, it is essential for efficient and robust apical constriction during ventral furrow formation.
Volume 15(3)
Pages 193-208
Published 2010-3
DOI 10.1111/j.1365-2443.2010.01389.x
PMID 20184661
MeSH Adherens Junctions / physiology Animals Cadherins / genetics Cadherins / metabolism* Drosophila / embryology Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Ectoderm / embryology Ectoderm / metabolism Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Epithelium / embryology Epithelium / metabolism Gastrulation Ovum / metabolism Protein Binding Protein Structure, Tertiary
IF 2.048
Times Cited 9