RRC ID |
11659
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著者 |
Haruta T, Warrior R, Yonemura S, Oda H.
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タイトル |
The proximal half of the Drosophila E-cadherin extracellular region is dispensable for many cadherin-dependent events but required for ventral furrow formation.
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ジャーナル |
Genes Cells
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Abstract |
The formation of the ventral furrow during Drosophila gastrulation is driven by coordinated apical constriction. Cell-cell adhesion is thought to regulate apical constriction, but the mechanisms are poorly understood. DE-cadherin, an epithelial classic cadherin, has in its membrane-proximal extracellular region a suite of domains absent from vertebrate/urochordate classic cadherins. We constructed DEΔP, a DE-cadherin derivative that lacks the membrane-proximal half of the extracellular region but retains the entire cytoplasmic domain and still exhibits strong cell-cell binding ability. The extracellular region of DEΔP consists of only cadherin repeats, mimicking vertebrate/urochordate classic cadherins. In animals lacking DE-cadherin, DEΔP organized adherens junction assembly and functioned fully in many cadherin-dependent processes, including oogenesis. Embryos in which DE-cadherin was entirely replaced by DEΔP established the blastoderm epithelium but failed to form a ventral furrow. Apical constrictions were initiated relatively normally but subsequently decelerated. These were then followed by catastrophic disruption of the junctional network. Our results suggest that although the membrane-proximal half of the DE-cadherin extracellular region is dispensable for many developmental events, it is essential for efficient and robust apical constriction during ventral furrow formation.
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巻・号 |
15(3)
|
ページ |
193-208
|
公開日 |
2010-3-1
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DOI |
10.1111/j.1365-2443.2010.01389.x
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PII |
GTC1389
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PMID |
20184661
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MeSH |
Adherens Junctions / physiology
Animals
Cadherins / genetics
Cadherins / metabolism*
Drosophila / embryology
Drosophila / metabolism*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Ectoderm / embryology
Ectoderm / metabolism
Embryo, Nonmammalian / embryology
Embryo, Nonmammalian / metabolism
Epithelium / embryology
Epithelium / metabolism
Gastrulation
Ovum / metabolism
Protein Binding
Protein Structure, Tertiary
|
IF |
1.655
|
引用数 |
9
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WOS 分野
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GENETICS & HEREDITY
CELL BIOLOGY
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リソース情報 |
ショウジョウバエ |
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