論文 - 詳細
| RRC ID | 11681 |
|---|---|
| 著者 | Rogers RL, Bedford T, Lyons AM, Hartl DL. |
| タイトル | Adaptive impact of the chimeric gene Quetzalcoatl in Drosophila melanogaster. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Chimeric genes, which form through the genomic fusion of two protein-coding genes, are a significant source of evolutionary novelty in Drosophila melanogaster. However, the propensity of chimeric genes to produce adaptive phenotypic changes is not fully understood. Here, we describe the chimeric gene Quetzalcoatl (Qtzl; CG31864), which formed in the recent past and swept to fixation in D. melanogaster. Qtzl arose through a duplication on chromosome 2L that united a portion of the mitochondrially targeted peptide CG12264 with a segment of the polycomb gene escl. The 3' segment of the gene, which is derived from escl, is inherited out of frame, producing a unique peptide sequence. Nucleotide diversity is drastically reduced and site frequency spectra are significantly skewed surrounding the duplicated region, a finding consistent with a selective sweep on the duplicate region containing Qtzl. Qtzl has an expression profile that largely resembles that of escl, with expression in early pupae, adult females, and male testes. However, expression patterns appear to have been decoupled from both parental genes during later embryonic development and in head tissues of adult males, indicating that Qtzl has developed a distinct regulatory profile through the rearrangement of different 5' and 3' regulatory domains. Furthermore, misexpression of Qtzl suppresses defects in the formation of the neuromuscular junction in larvae, demonstrating that Qtzl can produce phenotypic effects in cells. Together, these results show that chimeric genes can produce structural and regulatory changes in a single mutational step and may be a major factor in adaptive evolution. |
| 巻・号 | 107(24) |
| ページ | 10943-8 |
| 公開日 | 2010-6-15 |
| DOI | 10.1073/pnas.1006503107 |
| PII | 1006503107 |
| PMID | 20534482 |
| PMC | PMC2890713 |
| MeSH | Adaptation, Physiological / genetics Animals Base Sequence Chimera / genetics DNA Primers / genetics Drosophila Proteins / genetics Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / physiology Evolution, Molecular* Female Frameshift Mutation Gene Duplication Gene Expression Regulation, Developmental Gene Fusion Genes, Insect* Male Mutant Chimeric Proteins / genetics Phenotype Repressor Proteins / genetics |
| IF | 9.412 |
| 引用数 | 19 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#201346 |