RRC ID 11690
Author Tsurudome K, Tsang K, Liao EH, Ball R, Penney J, Yang JS, Elazzouzi F, He T, Chishti A, Lnenicka G, Lai EC, Haghighi AP.
Title The Drosophila miR-310 cluster negatively regulates synaptic strength at the neuromuscular junction.
Journal Neuron
Abstract Emerging data implicate microRNAs (miRNAs) in the regulation of synaptic structure and function, but we know little about their role in the regulation of neurotransmission in presynaptic neurons. Here, we demonstrate that the miR-310-313 cluster is required for normal synaptic transmission at the Drosophila larval neuromuscular junction. Loss of miR-310-313 cluster leads to a significant enhancement of neurotransmitter release, which can be rescued with temporally restricted expression of mir-310-313 in larval presynaptic neurons. Kinesin family member, Khc-73 is a functional target for miR-310-313 as its expression is increased in mir-310-313 mutants and reducing it restores normal synaptic function. Cluster mutants show an increase in the active zone protein Bruchpilot accompanied by an increase in electron dense T bars. Finally, we show that repression of Khc-73 by miR-310-313 cluster influences the establishment of normal synaptic homeostasis. Our findings establish a role for miRNAs in the regulation of neurotransmitter release.
Volume 68(5)
Pages 879-93
Published 2010-12-9
DOI 10.1016/j.neuron.2010.11.016
PII S0896-6273(10)00932-3
PMID 21145002
PMC PMC3034365
MeSH Animals Drosophila / genetics* Drosophila / growth & development Drosophila / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Kinesin / metabolism Larva / genetics Larva / metabolism MicroRNAs / genetics* Motor Neurons / metabolism Multigene Family / genetics Neuromuscular Junction / genetics* Neuromuscular Junction / metabolism Synaptic Transmission / genetics* Synaptic Transmission / physiology
IF 14.403
Times Cited 41