論文 - 詳細
| RRC ID | 11715 |
|---|---|
| 著者 | Pappu KS, Morey M, Nern A, Spitzweck B, Dickson BJ, Zipursky SL. |
| タイトル | Robo-3--mediated repulsive interactions guide R8 axons during Drosophila visual system development. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The formation of neuronal connections requires the precise guidance of developing axons toward their targets. In the Drosophila visual system, photoreceptor neurons (R cells) project from the eye into the brain. These cells are grouped into some 750 clusters comprised of eight photoreceptors or R cells each. R cells fall into three classes: R1 to R6, R7, and R8. Posterior R8 cells are the first to project axons into the brain. How these axons select a specific pathway is not known. Here, we used a microarray-based approach to identify genes expressed in R8 neurons as they extend into the brain. We found that Roundabout-3 (Robo3), an axon-guidance receptor, is expressed specifically and transiently in R8 growth cones. In wild-type animals, posterior-most R8 axons extend along a border of glial cells demarcated by the expression of Slit, the secreted ligand of Robo3. In contrast, robo3 mutant R8 axons extend across this border and fasciculate inappropriately with other axon tracts. We demonstrate that either Robo1 or Robo2 rescues the robo3 mutant phenotype when each is knocked into the endogenous robo3 locus separately, indicating that R8 does not require a function unique to the Robo3 paralog. However, persistent expression of Robo3 in R8 disrupts the layer-specific targeting of R8 growth cones. Thus, the transient cell-specific expression of Robo3 plays a crucial role in establishing neural circuits in the Drosophila visual system by selectively regulating pathway choice for posterior-most R8 growth cones. |
| 巻・号 | 108(18) |
| ページ | 7571-6 |
| 公開日 | 2011-5-3 |
| DOI | 10.1073/pnas.1103419108 |
| PII | 1103419108 |
| PMID | 21490297 |
| PMC | PMC3088640 |
| MeSH | Animals Axons / metabolism Axons / physiology* Drosophila / embryology* Drosophila Proteins / metabolism* Growth Cones / metabolism* Image Processing, Computer-Assisted Immunohistochemistry Microarray Analysis Nerve Tissue Proteins / metabolism Photoreceptor Cells, Invertebrate / metabolism Photoreceptor Cells, Invertebrate / physiology* Receptors, Immunologic / metabolism* Visual Pathways / embryology* |
| IF | 9.412 |
| 引用数 | 11 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |