RRC ID |
11766
|
著者 |
Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M.
|
タイトル |
The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos.
|
ジャーナル |
Nucleic Acids Res
|
Abstract |
Differences between male and female mammals are initiated by embryonic differentiation of the gonad into either a testis or an ovary. However, this may not be the sole determinant. There are reports that embryonic sex differentiation might precede and be independent of gonadal differentiation, but there is little molecular biological evidence for this. To test for sex differences in early-stage embryos, we separated male and female blastocysts using newly developed non-invasive sexing methods for transgenic mice expressing green fluorescent protein and compared the gene-expression patterns. From this screening, we found that the Fthl17 (ferritin, heavy polypeptide-like 17) family of genes was predominantly expressed in female blastocysts. This comprises seven genes that cluster on the X chromosome. Expression analysis based on DNA polymorphisms revealed that these genes are imprinted and expressed from the paternal X chromosome as early as the two-cell stage. Thus, by the time zygotic genome activation starts there are already differences in gene expression between male and female mouse embryos. This discovery will be important for the study of early sex differentiation, as clearly these differences arise before gonadal differentiation.
|
巻・号 |
38(11)
|
ページ |
3672-81
|
公開日 |
2010-6-1
|
DOI |
10.1093/nar/gkq113
|
PII |
gkq113
|
PMID |
20185572
|
PMC |
PMC2887969
|
MeSH |
Animals
Blastocyst / metabolism*
Embryo, Mammalian / metabolism
Female
Ferritins / genetics*
Gene Expression Profiling
Genomic Imprinting*
Male
Mice
Mice, Transgenic
Multigene Family*
Oligonucleotide Array Sequence Analysis
Sex Differentiation / genetics*
X Chromosome
X Chromosome Inactivation
|
IF |
11.502
|
引用数 |
22
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
実験動物マウス |
RBRC00980 |